The University of Southampton
University of Southampton Institutional Repository

Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts

Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts
Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts
The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.
115-122
Humana
Bhome, Rahul
d7b1e0d3-5925-460a-871d-5f52f69c649b
Goh, Rebecca
2dbe79e1-ec3d-4f2f-86bf-70f33269eab8
Pickard, Karen
e5188669-dff1-49c7-9c6f-8122b0c74bd9
Mellone, Massimiliano
b0301b32-14f8-4203-9026-b7f90885cab9
Sayan, A. Emre
d1dbbcad-9c53-47c1-8b7e-1b45cc56e077
Mirnezami, Alex
b3c7aee7-46a4-404c-bfe3-f72388e0bc94
Rani, S.
Bhome, Rahul
d7b1e0d3-5925-460a-871d-5f52f69c649b
Goh, Rebecca
2dbe79e1-ec3d-4f2f-86bf-70f33269eab8
Pickard, Karen
e5188669-dff1-49c7-9c6f-8122b0c74bd9
Mellone, Massimiliano
b0301b32-14f8-4203-9026-b7f90885cab9
Sayan, A. Emre
d1dbbcad-9c53-47c1-8b7e-1b45cc56e077
Mirnezami, Alex
b3c7aee7-46a4-404c-bfe3-f72388e0bc94
Rani, S.

Bhome, Rahul, Goh, Rebecca, Pickard, Karen, Mellone, Massimiliano, Sayan, A. Emre and Mirnezami, Alex (2017) Profiling the microRNA payload of exosomes derived from ex vivo primary colorectal fibroblasts. In, Rani, S. (ed.) MicroRNA Profiling. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-6524-3_11), 1509) New York. Humana, pp. 115-122. (doi:10.1007/978-1-4939-6524-3_11).

Record type: Book Section

Abstract

The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.

Full text not available from this repository.

More information

e-pub ahead of print date: 9 November 2016
Published date: 2017
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 405386
URI: https://eprints.soton.ac.uk/id/eprint/405386
PURE UUID: 8d637a28-1361-427e-8d1e-a525c260f4f5
ORCID for Massimiliano Mellone: ORCID iD orcid.org/0000-0002-4964-9340

Catalogue record

Date deposited: 03 Feb 2017 11:26
Last modified: 06 Jun 2018 12:34

Export record

Altmetrics

Contributors

Author: Rahul Bhome
Author: Rebecca Goh
Author: Karen Pickard
Author: Massimiliano Mellone ORCID iD
Author: A. Emre Sayan
Author: Alex Mirnezami
Editor: S. Rani

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×