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Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression

Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression
Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression
Understanding the passive mechanical properties of infarcted tissue at different healing stages is essential to explore the emerging biomaterial injection-based therapy for myocardial infarction (MI). Although rats have been widely used as animal models in such investigations, the data in literature that quantify the passive mechanical properties of rat heart infarcts is very limited. MI was induced in rats and hearts were harvested immediately (0 day), 7, 14 and 28 days after infarction onset. Left ventricle anterioapical samples were cut and underwent equibiaxial and non equibiaxial tension followed by uniaxial compression mechanical tests. Histological analysis was conducted to confirm MI and to quantify the size of the induced infarcts. Infarcts maintained anisotropy and the nonlinear biaxial and compressive mechanical behaviour throughout the healing phases with the circumferential direction being stiffer than the longitudinal direction. Mechanical coupling was observed between the two axes in all infarct groups. The 0, 7, 14 and 28 days infarcts showed 438, 693, 1048 and 1218 kPa circumferential tensile moduli. The 28 day infarct group showed a significantly higher compressive modulus compared to the other infarct groups (p=0.0060, 0.0293, and 0.0268 for 0, 7 and 14 days groups). Collagen fibres were found to align in a preferred direction for all infarct groups supporting the observed mechanical anisotropy. The presented data are useful for developing material models for healing infarcts and for setting a baseline for future assessment of emerging mechanical-based MI therapies.
1751-6161
262-264
Sirry, M.
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Butler, J.R.
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Patnaik, S.S.
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Brazile, B.
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Bertucci, R.
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Claude, A.
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McLaughlin, R.
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Davies, N.H.
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Liao, J.
1192392f-3b4e-4560-90d8-24264359661e
Franz, T.
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Sirry, M.
a176cdea-a811-437d-87ba-243fd9c0dd55
Butler, J.R.
6ef4f60a-2d11-4584-bab0-8e94dd678f3f
Patnaik, S.S.
06d9cfaa-0fb3-4b78-be53-19c05e792a6a
Brazile, B.
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Bertucci, R.
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Claude, A.
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McLaughlin, R.
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Davies, N.H.
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Liao, J.
1192392f-3b4e-4560-90d8-24264359661e
Franz, T.
d489e297-8608-4ec9-a428-ad31c7d15f05

Sirry, M., Butler, J.R., Patnaik, S.S., Brazile, B., Bertucci, R., Claude, A., McLaughlin, R., Davies, N.H., Liao, J. and Franz, T. (2016) Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression. Journal of the Mechanical Behavior of Biomedical Materials, 63, 262-264. (doi:10.1016/j.jmbbm.2016.06.029).

Record type: Article

Abstract

Understanding the passive mechanical properties of infarcted tissue at different healing stages is essential to explore the emerging biomaterial injection-based therapy for myocardial infarction (MI). Although rats have been widely used as animal models in such investigations, the data in literature that quantify the passive mechanical properties of rat heart infarcts is very limited. MI was induced in rats and hearts were harvested immediately (0 day), 7, 14 and 28 days after infarction onset. Left ventricle anterioapical samples were cut and underwent equibiaxial and non equibiaxial tension followed by uniaxial compression mechanical tests. Histological analysis was conducted to confirm MI and to quantify the size of the induced infarcts. Infarcts maintained anisotropy and the nonlinear biaxial and compressive mechanical behaviour throughout the healing phases with the circumferential direction being stiffer than the longitudinal direction. Mechanical coupling was observed between the two axes in all infarct groups. The 0, 7, 14 and 28 days infarcts showed 438, 693, 1048 and 1218 kPa circumferential tensile moduli. The 28 day infarct group showed a significantly higher compressive modulus compared to the other infarct groups (p=0.0060, 0.0293, and 0.0268 for 0, 7 and 14 days groups). Collagen fibres were found to align in a preferred direction for all infarct groups supporting the observed mechanical anisotropy. The presented data are useful for developing material models for healing infarcts and for setting a baseline for future assessment of emerging mechanical-based MI therapies.

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Sirry_et_al_2016_JMBBM manuscript.pdf - Accepted Manuscript
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Accepted/In Press date: 29 June 2016
e-pub ahead of print date: 5 July 2016
Published date: October 2016
Organisations: Bioengineering Group, nCATS Group

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Local EPrints ID: 405653
URI: http://eprints.soton.ac.uk/id/eprint/405653
ISSN: 1751-6161
PURE UUID: 09935296-3389-48ce-893c-068356fe5ac4

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Date deposited: 10 Feb 2017 10:31
Last modified: 15 Mar 2024 06:19

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Contributors

Author: M. Sirry
Author: J.R. Butler
Author: S.S. Patnaik
Author: B. Brazile
Author: R. Bertucci
Author: A. Claude
Author: R. McLaughlin
Author: N.H. Davies
Author: J. Liao
Author: T. Franz

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