Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm

Knap, Tea, Cregeen, Sara, Javornik, Branka, Hladnik, Matjaž and Bandelj, Dunja (2016) Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm. Brazilian Journal of Botany, 39 (4), 1095-1102. (doi:10.1007/s40415-016-0299-2).

Abstract

To support studies of the genetic diversity and population structure of Ficus carica L., 16 additional microsatellite markers were developed from genomic libraries enriched for GA/TC and GT/AC motifs. A total of 87 alleles were amplified in 83 analyzed fig samples. The number of alleles per locus ranged from three to eight, with a mean value of 5.44 alleles per locus. Mean values of observed and expected heterozygosity were 0.575 and 0.626, respectively. Calculated PIC values varied among loci in a range from 0.369 to 0.761. All markers were successfully cross-amplified in five closely related species belonging to the Moraceae family: Ficus (F. barteri Sprague, F. rubiginosa Desf. ex Vent., F. benjamina L.) and Morus (M. alba L. and M. nigra L.). These novel microsatellite markers represent a new valuable tool for further genome investigation, population studies, and management of genetic resources of wild, feral, and domesticated Ficus carica L. samples and other related species of Moraceae family, distributed mostly in tropical and subtropical regions throughout the world.

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