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Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm

Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm
Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm
To support studies of the genetic diversity and population structure of Ficus carica L., 16 additional microsatellite markers were developed from genomic libraries enriched for GA/TC and GT/AC motifs. A total of 87 alleles were amplified in 83 analyzed fig samples. The number of alleles per locus ranged from three to eight, with a mean value of 5.44 alleles per locus. Mean values of observed and expected heterozygosity were 0.575 and 0.626, respectively. Calculated PIC values varied among loci in a range from 0.369 to 0.761. All markers were successfully cross-amplified in five closely related species belonging to the Moraceae family: Ficus (F. barteri Sprague, F. rubiginosa Desf. ex Vent., F. benjamina L.) and Morus (M. alba L. and M. nigra L.). These novel microsatellite markers represent a new valuable tool for further genome investigation, population studies, and management of genetic resources of wild, feral, and domesticated Ficus carica L. samples and other related species of Moraceae family, distributed mostly in tropical and subtropical regions throughout the world.
Cross-amplification, Moraceae, Morus sp., Primers
0100-8404
1095-1102
Knap, Tea
ccbab1ff-8b82-496e-92a8-2f51b15e02a5
Cregeen, Sara
3f61d96e-0e60-4a16-84d3-059489c9f192
Javornik, Branka
0fad8c7e-14ee-4615-a4b8-265bfbb9264d
Hladnik, Matjaž
c8434571-9241-46f0-bb0a-b3611eb5fd0d
Bandelj, Dunja
92ac81c6-7a40-40ee-8297-5a9a84ca6638
Knap, Tea
ccbab1ff-8b82-496e-92a8-2f51b15e02a5
Cregeen, Sara
3f61d96e-0e60-4a16-84d3-059489c9f192
Javornik, Branka
0fad8c7e-14ee-4615-a4b8-265bfbb9264d
Hladnik, Matjaž
c8434571-9241-46f0-bb0a-b3611eb5fd0d
Bandelj, Dunja
92ac81c6-7a40-40ee-8297-5a9a84ca6638

Knap, Tea, Cregeen, Sara, Javornik, Branka, Hladnik, Matjaž and Bandelj, Dunja (2016) Characterization and defining of a core set of novel microsatellite markers for use in genotyping and diversity study of Adriatic fig (Ficus carica L.) germplasm. Brazilian Journal of Botany, 39 (4), 1095-1102. (doi:10.1007/s40415-016-0299-2).

Record type: Article

Abstract

To support studies of the genetic diversity and population structure of Ficus carica L., 16 additional microsatellite markers were developed from genomic libraries enriched for GA/TC and GT/AC motifs. A total of 87 alleles were amplified in 83 analyzed fig samples. The number of alleles per locus ranged from three to eight, with a mean value of 5.44 alleles per locus. Mean values of observed and expected heterozygosity were 0.575 and 0.626, respectively. Calculated PIC values varied among loci in a range from 0.369 to 0.761. All markers were successfully cross-amplified in five closely related species belonging to the Moraceae family: Ficus (F. barteri Sprague, F. rubiginosa Desf. ex Vent., F. benjamina L.) and Morus (M. alba L. and M. nigra L.). These novel microsatellite markers represent a new valuable tool for further genome investigation, population studies, and management of genetic resources of wild, feral, and domesticated Ficus carica L. samples and other related species of Moraceae family, distributed mostly in tropical and subtropical regions throughout the world.

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More information

e-pub ahead of print date: 29 June 2016
Published date: 1 December 2016
Keywords: Cross-amplification, Moraceae, Morus sp., Primers
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 405801
URI: http://eprints.soton.ac.uk/id/eprint/405801
ISSN: 0100-8404
PURE UUID: 3b8fbd7b-8eb5-4b9f-a78e-bbedde354a80

Catalogue record

Date deposited: 10 Feb 2017 14:28
Last modified: 08 Jan 2022 06:41

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Contributors

Author: Tea Knap
Author: Sara Cregeen
Author: Branka Javornik
Author: Matjaž Hladnik
Author: Dunja Bandelj

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