Electroformation of giant unilamellar vesicles on stainless steel electrodes
Electroformation of giant unilamellar vesicles on stainless steel electrodes
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively mono-disperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared to other methods. In this study, we demonstrate that 1,2-dioleoyl-snglycero-3-phosphatidylcholine (DOPC) GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.
Electroformation, Electroswelling, Giant unilamellar vesicles, Stainless steel electrode, Platinum wire, Generalized polarization, Lipid packing, DOPC, Lipid oxidation, Lipid hydrolysis
994-1002
Pereno, Valerio
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Carugo, Dario
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Bau, Luca
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Sezgin, Erdinc
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Bernardino de la Serna, Jorge
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Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Stride, Eleanor
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2017
Pereno, Valerio
3106035d-e7d9-4af8-af59-dd5091532ea7
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Bau, Luca
7ab4b1fb-e69a-4a53-8275-aceac2c0d264
Sezgin, Erdinc
7b0b5507-2421-46f8-8ef0-04a7bc510348
Bernardino de la Serna, Jorge
d568eb8d-3a54-4fa0-bbf0-7429300aa31f
Eggeling, Christian
8003f376-30f6-49b4-bb50-bab8545b5ffd
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Pereno, Valerio, Carugo, Dario, Bau, Luca, Sezgin, Erdinc, Bernardino de la Serna, Jorge, Eggeling, Christian and Stride, Eleanor
(2017)
Electroformation of giant unilamellar vesicles on stainless steel electrodes.
ACS Omega, 2 (3), .
(doi:10.1021/acsomega.6b00395).
Abstract
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively mono-disperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared to other methods. In this study, we demonstrate that 1,2-dioleoyl-snglycero-3-phosphatidylcholine (DOPC) GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.
Text
VPereno-ACS Omega-2017
- Accepted Manuscript
Text
VPereno-ACS Omega-2017
- Version of Record
More information
Accepted/In Press date: 3 March 2017
e-pub ahead of print date: 16 March 2017
Published date: 2017
Keywords:
Electroformation, Electroswelling, Giant unilamellar vesicles, Stainless steel electrode, Platinum wire, Generalized polarization, Lipid packing, DOPC, Lipid oxidation, Lipid hydrolysis
Organisations:
Bioengineering Group
Identifiers
Local EPrints ID: 406190
URI: http://eprints.soton.ac.uk/id/eprint/406190
ISSN: 2470-1343
PURE UUID: 2957ee9b-812e-4b0e-b276-bd6c3780922e
Catalogue record
Date deposited: 10 Mar 2017 10:41
Last modified: 16 Mar 2024 05:06
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Contributors
Author:
Valerio Pereno
Author:
Luca Bau
Author:
Erdinc Sezgin
Author:
Jorge Bernardino de la Serna
Author:
Christian Eggeling
Author:
Eleanor Stride
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