The University of Southampton
University of Southampton Institutional Repository

Role of single nucleotide polymorphisms of pro-inflammatory cytokine genes in the relationship between serum lipids and inflammatory parameters, and the lipid-lowering effect of fish oil in healthy males

Markovic, Olivera, O'Reilly, Gillian, Fussell, Helen M., Turner, Stephen J., Calder, Philip C., Howell, William M. and Grimble, Robert F. (2004) Role of single nucleotide polymorphisms of pro-inflammatory cytokine genes in the relationship between serum lipids and inflammatory parameters, and the lipid-lowering effect of fish oil in healthy males Clinical Nutrition, 23, (5), pp. 1084-1095. (doi:10.1016/j.clnu.2004.02.002).

Record type: Article

Abstract

Background and Aims: Lipid metabolism, obesity and inflammation are intimately related. Plasma triglycerides increase during the inflammatory response to pathogens and obesity increases inflammatory stress.
Pro-inflammatory cytokines are secreted by adipocytes in uninfected obese subjects. Polymorphisms (SNPs) in cytokine genes influence the intensity of cytokine production and inflammatory stress. Fish oil has lipid-lowering and anti-inflammatory properties. The influence of cytokine gene polymorphisms on the interaction between adiposity, inflammation and the properties of fish oil is unknown.
Methods: Fasting plasma triglycerides, acute phase proteins and BMI were studied in 159 healthy men and the effect of 6 g/d fish oil for 12 weeks on the former two parameters studied. Subjects were genotyped for SNPs at positions ?511, ?174, +252 and ?308 in the IL-1?, IL-6, LT-? (TNF-?) and TNF-? genes, respectively. Data were divided into three sub?groups of BMI, 16.7–22.8, 22.9–24.9 and 25.1–33.7 kg/m2, respectively.
Results:Correlations were apparent between CRP and triglycerides in the highest tertile r=0.324, P<0.05 and between CRP and serum amyloid in all tertiles. Mean concentrations of all three molecules were higher in the middle and highest tertile than in the lowest. Irrespective of BMI, CRP and triglycerides were positively correlated in subjects with a TNF-??308GG, LT-? AG, IL-1??511TT and IL-6?174GG genotype. The latter three genotypes are associated with enhanced inflammation. Genotype and BMI interacted. Concentrations of triglyceride rose significantly with increasing tertile only in subjects with a LT-? AA genotype. CRP concentrations rose in subjects with a LT-? AG genotype. Triglycerides were lowered by fish oil. Pre?supplementation concentrations were correlated with the decrease, r=?0.494 P<0.0001. Genotype influenced the effects of fish oil. A fall occurred in triglycerides, across tertiles of BMI, only in individuals possessing a LT-?+252 AA genotype. Irrespective of BMI, possession of an A allele of this SNP was necessary for the correlation to occur.
Conclusions: Possession of genotypes associated with raised inflammatory stress strengthen the association between fasting plasma triglycerides and CRP.
The ability of fish oil to exert a lipid-lowering, anti?inflammatory influence in healthy men is influenced by BMI and possession of the LT-?+252 A allele.

Full text not available from this repository.

More information

Published date: 2004
Keywords: fish oil, cytokines, gene polymorphisms, tnf-?, lt-?, Il-1?, il-6

Identifiers

Local EPrints ID: 40640
URI: http://eprints.soton.ac.uk/id/eprint/40640
ISSN: 0261-5614
PURE UUID: d672f95d-d290-4870-874a-a4ed72ac1474

Catalogue record

Date deposited: 07 Jul 2006
Last modified: 16 Apr 2017 21:51

Export record

Altmetrics

Contributors

Author: Olivera Markovic
Author: Helen M. Fussell
Author: Stephen J. Turner
Author: William M. Howell
Author: Robert F. Grimble

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×