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Progressive metabolic impairment underlies the novel nematicidal action of fluensulfone on the potato cyst nematode Globodera pallida

Progressive metabolic impairment underlies the novel nematicidal action of fluensulfone on the potato cyst nematode Globodera pallida
Progressive metabolic impairment underlies the novel nematicidal action of fluensulfone on the potato cyst nematode Globodera pallida
Background: Fluensulfone is a new nematicide with an excellent profile of selective toxicity against plant parasitic nematodes. Here, its effects on the physiology and biochemistry of the potato cyst nematode Globodera pallida have been investigated and comparisons made with its effect on the life-span of the free-living nematode Caenorhabditis elegans to provide insight into its mode of action and its selective toxicity.

Results: Fluensulfone exerts acute effects (≤ 1 h; ≥ 100 μM) on stylet thrusting and motility of hatched second stage G. pallida juveniles (J2s). Chronic exposure to lower concentrations of fluensulfone (≥ 3 days; ≤ 30 μM), reveals a slowly developing metabolic insult in which G. pallida J2s sequentially exhibit a reduction in motility, loss of a metabolic marker for cell viability, high lipid content and tissue degeneration prior to death. These effects are absent in adults and dauers of the model genetic nematode Caenorhabditis elegans.

Conclusion: The nematicidal action of fluensulfone follows a time-course which progresses from an early impact on motility through to an accumulating metabolic impairment, an inability to access lipid stores and death.
0048-3575
Kearn, James
555068e2-ab5c-4f57-a63c-4e20b1d22428
Lilley, Catherine
032c7c73-a779-4784-8686-00d22245be32
Unwin, Peter
2727d3f2-b0aa-40bd-a9e8-65bf8dd30781
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
Holden-Dye, Linda
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Kearn, James
555068e2-ab5c-4f57-a63c-4e20b1d22428
Lilley, Catherine
032c7c73-a779-4784-8686-00d22245be32
Unwin, Peter
2727d3f2-b0aa-40bd-a9e8-65bf8dd30781
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
Holden-Dye, Linda
8032bf60-5db6-40cb-b71c-ddda9d212c8e

Kearn, James, Lilley, Catherine, Unwin, Peter, O'Connor, Vincent and Holden-Dye, Linda (2017) Progressive metabolic impairment underlies the novel nematicidal action of fluensulfone on the potato cyst nematode Globodera pallida. Pesticide Biochemistry and Physiology. (doi:10.1016/j.pestbp.2017.01.009).

Record type: Article

Abstract

Background: Fluensulfone is a new nematicide with an excellent profile of selective toxicity against plant parasitic nematodes. Here, its effects on the physiology and biochemistry of the potato cyst nematode Globodera pallida have been investigated and comparisons made with its effect on the life-span of the free-living nematode Caenorhabditis elegans to provide insight into its mode of action and its selective toxicity.

Results: Fluensulfone exerts acute effects (≤ 1 h; ≥ 100 μM) on stylet thrusting and motility of hatched second stage G. pallida juveniles (J2s). Chronic exposure to lower concentrations of fluensulfone (≥ 3 days; ≤ 30 μM), reveals a slowly developing metabolic insult in which G. pallida J2s sequentially exhibit a reduction in motility, loss of a metabolic marker for cell viability, high lipid content and tissue degeneration prior to death. These effects are absent in adults and dauers of the model genetic nematode Caenorhabditis elegans.

Conclusion: The nematicidal action of fluensulfone follows a time-course which progresses from an early impact on motility through to an accumulating metabolic impairment, an inability to access lipid stores and death.

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Accepted/In Press date: 12 January 2017
e-pub ahead of print date: 13 January 2017
Organisations: Biomedicine, Centre for Biological Sciences

Identifiers

Local EPrints ID: 406428
URI: http://eprints.soton.ac.uk/id/eprint/406428
ISSN: 0048-3575
PURE UUID: 629d087b-79aa-4b2d-b787-c7ed13ed7ddc
ORCID for Vincent O'Connor: ORCID iD orcid.org/0000-0003-3185-5709
ORCID for Linda Holden-Dye: ORCID iD orcid.org/0000-0002-9704-1217

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Date deposited: 10 Mar 2017 10:47
Last modified: 16 Mar 2024 03:13

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Contributors

Author: James Kearn
Author: Catherine Lilley
Author: Peter Unwin

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