Identification of splicing silencers and enhancers in sense Alus: a role for pseudoacceptors in splice site repression
Identification of splicing silencers and enhancers in sense Alus: a role for pseudoacceptors in splice site repression
Auxiliary splicing signals in introns play an important role in splice site selection, but these elements are poorly understood. We show that a subset of serine/arginine (SR)-rich proteins activate a cryptic 3' splice site in a sense Alu repeat located in intron 4 of the human LST1 gene. Utilization of this cryptic splice site is controlled by juxtaposed Alu-derived splicing silencers and enhancers between closely linked short tandem repeats TNFd and TNFe. Systematic mutagenesis of these elements showed that AG dinucleotides that were not preceded by purine residues were critical for repressing exon inclusion of a chimeric splicing reporter. Since the splice acceptor-like sequences are present in excess in exonic splicing silencers, these signals may contribute to inhibition of a large number of pseudosites in primate genomes.
6912-6920
Lei, Haixin
24595b39-a4e6-4cfc-9354-5001b7f70d5a
Vořechovský, Igor
7245de2f-8c9b-4034-8935-9a451d9b682e
2005
Lei, Haixin
24595b39-a4e6-4cfc-9354-5001b7f70d5a
Vořechovský, Igor
7245de2f-8c9b-4034-8935-9a451d9b682e
Lei, Haixin and Vořechovský, Igor
(2005)
Identification of splicing silencers and enhancers in sense Alus: a role for pseudoacceptors in splice site repression.
Molecular and Cellular Biology, 25 (16), .
(doi:10.1128/MCB.25.16.6912-6920.2005).
Abstract
Auxiliary splicing signals in introns play an important role in splice site selection, but these elements are poorly understood. We show that a subset of serine/arginine (SR)-rich proteins activate a cryptic 3' splice site in a sense Alu repeat located in intron 4 of the human LST1 gene. Utilization of this cryptic splice site is controlled by juxtaposed Alu-derived splicing silencers and enhancers between closely linked short tandem repeats TNFd and TNFe. Systematic mutagenesis of these elements showed that AG dinucleotides that were not preceded by purine residues were critical for repressing exon inclusion of a chimeric splicing reporter. Since the splice acceptor-like sequences are present in excess in exonic splicing silencers, these signals may contribute to inhibition of a large number of pseudosites in primate genomes.
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Published date: 2005
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Local EPrints ID: 40657
URI: http://eprints.soton.ac.uk/id/eprint/40657
ISSN: 0270-7306
PURE UUID: 48fe8a56-266f-4a26-813c-c59524d12cc5
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Date deposited: 07 Jul 2006
Last modified: 16 Mar 2024 03:32
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Author:
Haixin Lei
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