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HY peptides modulate transplantation responses to skin allografts

HY peptides modulate transplantation responses to skin allografts
HY peptides modulate transplantation responses to skin allografts
Injection of female C57BL/6 mice with immature female bone marrow-derived dendritic cells (BMDC) pulsed with a single immunodominant HYDb Uty peptide, WMHHNMDLI, induces prolonged survival of syngeneic male skin grafts. In contrast, injection of immature female BMDC pulsed with a single MHC class I-restricted HYAb Dby peptide, NAGFNSNRANSSRSS, causes immunization similar to that following injection of male cells. Tolerance induced by HYDb Uty peptide pretreatment is not characterized by clonal deletion: long-term tolerant mice maintain circulating HYDb Uty tetramer+ T cells which expand following exposure to male cells in vivo or in vitro. Tolerance to male skin grafts can be adoptively transferred into neonatal females with splenocytes from tolerant donors. Tolerance is specific—third-party skin grafts are rejected. We propose that tolerance in this model is initiated by cognate interaction of HYDb Uty-specific CD8+ T cells with their ligand, presented either on the injected immature BMDC or on recipient DC. This interaction leads to incomplete activation of the CD8+ T cells resulting in diminished responsiveness of CD4+ and CD8+ T cells specific for HY peptide epitopes subsequently presented on the male graft.
dendritic cell, epitope, immunoregulation, tetramer analysis, tolerance
0953-8178
1333-1342
James, E.
7dc1afb7-d326-4050-89fc-1f4e2a1a19a4
Scott, D.
4f3dd604-c3fc-4051-b81c-87ccdbe1ca43
Chai, J-G.
9030b9b3-e959-4184-99f9-afe4afa81a83
Millrain, M.
083b10e7-cd6f-436c-9c6e-f1d387b5f507
Chandler, P.
409b4218-39e2-4f55-8719-3bd018be42f2
Simpson, E.
127514a1-9431-48d2-b3dc-ff871a5e4155
James, E.
7dc1afb7-d326-4050-89fc-1f4e2a1a19a4
Scott, D.
4f3dd604-c3fc-4051-b81c-87ccdbe1ca43
Chai, J-G.
9030b9b3-e959-4184-99f9-afe4afa81a83
Millrain, M.
083b10e7-cd6f-436c-9c6e-f1d387b5f507
Chandler, P.
409b4218-39e2-4f55-8719-3bd018be42f2
Simpson, E.
127514a1-9431-48d2-b3dc-ff871a5e4155

James, E., Scott, D., Chai, J-G., Millrain, M., Chandler, P. and Simpson, E. (2002) HY peptides modulate transplantation responses to skin allografts. International Immunology, 14 (11), 1333-1342. (doi:10.1093/intimm/dxf093).

Record type: Article

Abstract

Injection of female C57BL/6 mice with immature female bone marrow-derived dendritic cells (BMDC) pulsed with a single immunodominant HYDb Uty peptide, WMHHNMDLI, induces prolonged survival of syngeneic male skin grafts. In contrast, injection of immature female BMDC pulsed with a single MHC class I-restricted HYAb Dby peptide, NAGFNSNRANSSRSS, causes immunization similar to that following injection of male cells. Tolerance induced by HYDb Uty peptide pretreatment is not characterized by clonal deletion: long-term tolerant mice maintain circulating HYDb Uty tetramer+ T cells which expand following exposure to male cells in vivo or in vitro. Tolerance to male skin grafts can be adoptively transferred into neonatal females with splenocytes from tolerant donors. Tolerance is specific—third-party skin grafts are rejected. We propose that tolerance in this model is initiated by cognate interaction of HYDb Uty-specific CD8+ T cells with their ligand, presented either on the injected immature BMDC or on recipient DC. This interaction leads to incomplete activation of the CD8+ T cells resulting in diminished responsiveness of CD4+ and CD8+ T cells specific for HY peptide epitopes subsequently presented on the male graft.

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More information

Published date: 2002
Keywords: dendritic cell, epitope, immunoregulation, tetramer analysis, tolerance

Identifiers

Local EPrints ID: 40678
URI: http://eprints.soton.ac.uk/id/eprint/40678
ISSN: 0953-8178
PURE UUID: e0962ccc-a350-4bfd-87e2-68ffae86dc0a

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Date deposited: 07 Jul 2006
Last modified: 15 Jul 2019 18:59

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