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Vaccination of human subjects expands both specific and bystander memory T cells, but antibody production remains vaccine-specific

Vaccination of human subjects expands both specific and bystander memory T cells, but antibody production remains vaccine-specific
Vaccination of human subjects expands both specific and bystander memory T cells, but antibody production remains vaccine-specific
Human subjects maintain long-term immunologic memory against infective organisms but the mechanism is unclear. CD4+ T-helper memory (Thmem) cells are pivotal in controlling humoral and cellular responses, therefore their longevity and response to vaccination are critical for maintenance of protective immunity.
To probe the dynamics of the Thmem-cell response to antigenic challenge, we investigated subjects following a booster injection with tetanus toxoid (TT). Expansion of TT-specific Thmem cells and cytokine production showed complex kinetics. Strikingly, parallel expansion and cytokine production occurred in pre-existing Thmem cells specific for 2 other common antigens: purified protein derivative of tuberculin and Candida albicans.
Bystander expansion occurred in Thmem but not in Thnaive cells. Antibody production against TT peaked approximately 2 weeks after vaccination and gradually declined. However, pre-existing antibody against the other antigens did not change. It appears that although all Thmem cells are readily stimulated to expand, antibody responses are controlled by antigen availability. These findings relate to the maintenance of memory and have consequences for assessments of specific T-cell responses to vaccination.
elispot assay, cells, immunity, helper-cells, t-cell, maintenance, time, vaccine, vaccination, t cells, antigen, mechanism, immunological memory, responses, cell, smallpox vaccination, protein, t-cells, naive, plasma-cells, expansion, protective immunity, activation
0006-4971
2806-2813
Di Genova, Gianfranco
c3b15017-39f0-4d9d-8b55-bb1da7358468
Roddick, Joanna
997137aa-b516-4c01-9f0b-1dc15994bc80
McNicholl, Feargal
05347c54-3757-4553-a202-06dd10a570be
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Di Genova, Gianfranco
c3b15017-39f0-4d9d-8b55-bb1da7358468
Roddick, Joanna
997137aa-b516-4c01-9f0b-1dc15994bc80
McNicholl, Feargal
05347c54-3757-4553-a202-06dd10a570be
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c

Di Genova, Gianfranco, Roddick, Joanna, McNicholl, Feargal and Stevenson, Freda K. (2006) Vaccination of human subjects expands both specific and bystander memory T cells, but antibody production remains vaccine-specific. Blood, 107 (7), 2806-2813. (doi:10.1182/blood-2005-08-3255).

Record type: Article

Abstract

Human subjects maintain long-term immunologic memory against infective organisms but the mechanism is unclear. CD4+ T-helper memory (Thmem) cells are pivotal in controlling humoral and cellular responses, therefore their longevity and response to vaccination are critical for maintenance of protective immunity.
To probe the dynamics of the Thmem-cell response to antigenic challenge, we investigated subjects following a booster injection with tetanus toxoid (TT). Expansion of TT-specific Thmem cells and cytokine production showed complex kinetics. Strikingly, parallel expansion and cytokine production occurred in pre-existing Thmem cells specific for 2 other common antigens: purified protein derivative of tuberculin and Candida albicans.
Bystander expansion occurred in Thmem but not in Thnaive cells. Antibody production against TT peaked approximately 2 weeks after vaccination and gradually declined. However, pre-existing antibody against the other antigens did not change. It appears that although all Thmem cells are readily stimulated to expand, antibody responses are controlled by antigen availability. These findings relate to the maintenance of memory and have consequences for assessments of specific T-cell responses to vaccination.

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More information

Published date: 2006
Keywords: elispot assay, cells, immunity, helper-cells, t-cell, maintenance, time, vaccine, vaccination, t cells, antigen, mechanism, immunological memory, responses, cell, smallpox vaccination, protein, t-cells, naive, plasma-cells, expansion, protective immunity, activation

Identifiers

Local EPrints ID: 40688
URI: http://eprints.soton.ac.uk/id/eprint/40688
ISSN: 0006-4971
PURE UUID: 98da989c-1b5a-4f29-ae0a-a6f601499dcc
ORCID for Freda K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

Catalogue record

Date deposited: 06 Jul 2006
Last modified: 26 Nov 2019 01:59

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Contributors

Author: Gianfranco Di Genova
Author: Joanna Roddick
Author: Feargal McNicholl

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