The University of Southampton
University of Southampton Institutional Repository

A Molecular Mousetrap Determines Polarity of Termination of DNA Replication in E. coli

Mulcair, M.D., Schaeffer, P.M., Oakley, A.J., Cross, H.F., Neylon, D.C., Hill, T.M. and Dixon, N.E. (2006) A Molecular Mousetrap Determines Polarity of Termination of DNA Replication in E. coli Cell, 125, (7), pp. 1309-1319. (doi:10.1016/j.cell.2006.04.040).

Record type: Article

Abstract

During chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound Ter sites on approach from one direction but not the other. To study the basis of this polarity, we measured the rates of dissociation of Tus from forked TerB oligonucleotides, such as would be produced by the replicative DnaB helicase at both the fork-blocking (nonpermissive) and permissive ends of the Ter site. Strand separation of a few nucleotides at the permissive end was sufficient to force rapid dissociation of Tus to allow fork progression. In contrast, strand separation extending to and including the strictly conserved G-C(6) base pair at the nonpermissive end led to formation of a stable locked complex. Lock formation specifically requires the cytosine residue, C(6). The crystal structure of the locked complex showed that C(6) moves 14 Å from its normal position to bind in a cytosine-specific pocket on the surface of Tus.

Full text not available from this repository.

More information

Submitted date: 19 December 2005
Published date: 30 June 2006

Identifiers

Local EPrints ID: 40707
URI: http://eprints.soton.ac.uk/id/eprint/40707
ISSN: 0092-8674
PURE UUID: f2db24a7-efd3-4cff-b3c1-f55b83900a57

Catalogue record

Date deposited: 15 Feb 2007
Last modified: 17 Jul 2017 15:33

Export record

Altmetrics

Contributors

Author: M.D. Mulcair
Author: P.M. Schaeffer
Author: A.J. Oakley
Author: H.F. Cross
Author: D.C. Neylon
Author: T.M. Hill
Author: N.E. Dixon

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×