Read me file: University of Southampton – ECS – Dr. Daniel Evans – Email: dan_evans@outlook.com The 5 separate .csv files contain experimental data for the paper. More specifically: -- Figure 2 SPR Data: Characterisation of antibody-target interaction using Surface Plasmon Resonance. Figure 3 Sensor Characterisation Data: Transient sensors behaviour using a TMB electrolyte. Figure 4 TMB Data: Comparison of amperometric and colorimetric detection of TMB product concentrations. A range of TMB product concentrations were achieved by allowing a range of enzymatic conversion times using a single HRP enzyme concentration. Results were obtained using three different amperometric sensors, however, all colorimetric results were obtained using the same colorimetric quantification apparatus (Promega Glomax). Figure 5 Assay/High Sensitivity Assay Data: Amperometric assay experiment using the modified assay components measured with the PCB-based sensor. (a) Amperometric measurement using the PCB-based sensor and in-house bioinstrumentation platform. (b) Colorimetric validation of the experimental results shown in (a). (a) and (b) show three discrete IFNgamma titration assays as described in Figure 1 with amperometric values measured on three discrete sensors and colorimetric values measured on one sensor (Promega Glomax). (c) Amperometric data for low concentration IFNgamma assay using Log_(1.5) analyte dilutions. (d) Colorimetric verification of the experimental results shown in (c). Figure 6 Assay in Plasma Data: Three discrete IFNgamma titration assays were performed in a plasma matrix. TMB reporter solutions harvested from each were applied to a different sensor to produce the three measurement series. -- Date of data collection: from May 2016 - September 2016 Information about geographic location of data collection: University of Southampton, U.K. Date that the file was created: March 2017