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MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells

MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells
MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells

Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs against human DROSHA and DICER1 or miRNA mimics or inhibitors. Wounding assays were performed and the cells were observed using time-lapse microscopy. The area of damage was calculated at chosen time points, followed by data analysis. Cells with silenced global miRNA expression showed a significantly slower repair rate compared to the control cells (p=0.001). For miR-328, we observed significantly delayed repair in cells transfected with the inhibitor compared to control (p=0.02). Global microRNA silencing significantly decreased the repair rate of airway epithelial cells in vitro, indicating an important role of miRNA in the regulation of wound repair and that miR-328, possibly involved in actin pathway, may be a potent modifier of this process.

Journal Article
1569-9048
59-65
Narożna, Beata
6b7fa4fb-58a3-4733-897e-be2ec6cc2c54
Langwinski, Wojciech
f9de82f2-03f0-4b85-b70e-5d2433bed346
Jackson, Claire
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Lackie, Peter
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Szczepankiewicz, Aleksandra
54b47a55-9a85-44c8-b4b9-dea4a8d9fe73
Narożna, Beata
6b7fa4fb-58a3-4733-897e-be2ec6cc2c54
Langwinski, Wojciech
f9de82f2-03f0-4b85-b70e-5d2433bed346
Jackson, Claire
64cdd6fa-74c3-4ac6-94ef-070620a6efd9
Lackie, Peter
4afbbe1a-22a6-4ceb-8cad-f3696dc43a7a
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Szczepankiewicz, Aleksandra
54b47a55-9a85-44c8-b4b9-dea4a8d9fe73

Narożna, Beata, Langwinski, Wojciech, Jackson, Claire, Lackie, Peter, Holloway, John W. and Szczepankiewicz, Aleksandra (2017) MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells. Respiratory Physiology & Neurobiology, 242, 59-65. (doi:10.1016/j.resp.2017.03.010).

Record type: Article

Abstract

Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs against human DROSHA and DICER1 or miRNA mimics or inhibitors. Wounding assays were performed and the cells were observed using time-lapse microscopy. The area of damage was calculated at chosen time points, followed by data analysis. Cells with silenced global miRNA expression showed a significantly slower repair rate compared to the control cells (p=0.001). For miR-328, we observed significantly delayed repair in cells transfected with the inhibitor compared to control (p=0.02). Global microRNA silencing significantly decreased the repair rate of airway epithelial cells in vitro, indicating an important role of miRNA in the regulation of wound repair and that miR-328, possibly involved in actin pathway, may be a potent modifier of this process.

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1-s2.0-S1569904817300952-main - Accepted Manuscript
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More information

Accepted/In Press date: 24 March 2017
e-pub ahead of print date: 25 March 2017
Published date: August 2017
Additional Information: Copyright © 2017. Published by Elsevier B.V.
Keywords: Journal Article
Organisations: Human Development & Health, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 407378
URI: https://eprints.soton.ac.uk/id/eprint/407378
ISSN: 1569-9048
PURE UUID: 21383340-c6fe-422e-8de3-063037c9c24f
ORCID for Peter Lackie: ORCID iD orcid.org/0000-0001-7138-3764
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 05 Apr 2017 01:05
Last modified: 19 Nov 2019 06:13

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