Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9
Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9
Bag1 transcribes a multifunctional protein that participates in many important biological processes such as cell apoptosis, proliferation, differentiation and embryo development. Despite numerous published studies, the role of Bag1 in the context of embryonic stem (ES) cells, has not been explored. To investigate the function of Bag1 in ES cells, we generated mutant Bag1−/− ES cells using the CRISPR/Cas9 system. We established that the Bag1 double knockout ES cell line maintained their pluripotency, possessed a normal karyotype and the ability to differentiate into all three germ layers.
29-31
Tang, Cheng-cheng
9e192ecb-4a9d-4fd6-906d-9c544e19032b
Shan, Li Pik
cbd41ae1-3034-4759-8af1-48c3a8aa81f9
Wang, Wu-ming
ebd5a012-b85d-4c4f-ae2f-f587a23f0800
Lu, Gang
b1155f9b-16f9-4117-a052-f1d026b0fc1c
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Lee, Kenneth Ka Ho
6f3bd64a-6369-4251-aacd-9405d49739a2
May 2017
Tang, Cheng-cheng
9e192ecb-4a9d-4fd6-906d-9c544e19032b
Shan, Li Pik
cbd41ae1-3034-4759-8af1-48c3a8aa81f9
Wang, Wu-ming
ebd5a012-b85d-4c4f-ae2f-f587a23f0800
Lu, Gang
b1155f9b-16f9-4117-a052-f1d026b0fc1c
Tare, Rahul
587c9db4-e409-4e7c-a02a-677547ab724a
Lee, Kenneth Ka Ho
6f3bd64a-6369-4251-aacd-9405d49739a2
Tang, Cheng-cheng, Shan, Li Pik, Wang, Wu-ming, Lu, Gang, Tare, Rahul and Lee, Kenneth Ka Ho
(2017)
Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9.
Stem Cell Research, 21, .
(doi:10.1016/j.scr.2017.03.016).
Abstract
Bag1 transcribes a multifunctional protein that participates in many important biological processes such as cell apoptosis, proliferation, differentiation and embryo development. Despite numerous published studies, the role of Bag1 in the context of embryonic stem (ES) cells, has not been explored. To investigate the function of Bag1 in ES cells, we generated mutant Bag1−/− ES cells using the CRISPR/Cas9 system. We established that the Bag1 double knockout ES cell line maintained their pluripotency, possessed a normal karyotype and the ability to differentiate into all three germ layers.
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Accepted_Tang_et_al_Stem_Cell_Research
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Accepted/In Press date: 20 March 2017
e-pub ahead of print date: 23 March 2017
Published date: May 2017
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 407419
URI: http://eprints.soton.ac.uk/id/eprint/407419
ISSN: 1873-5061
PURE UUID: 6479224e-a60f-443e-b940-ca93e47d5b8b
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Date deposited: 06 Apr 2017 01:03
Last modified: 16 Mar 2024 05:14
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Contributors
Author:
Cheng-cheng Tang
Author:
Li Pik Shan
Author:
Wu-ming Wang
Author:
Gang Lu
Author:
Kenneth Ka Ho Lee
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