Integrated single- and two-photon light sheet microscopy using accelerating beams
Integrated single- and two-photon light sheet microscopy using accelerating beams
We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
1-8
Piksarv, Peeter
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Marti, Dominik
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Le, Tuan
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Unterhuber, Angelika
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Forbes, Lindsey
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Andrews, Melissa R.
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Stingl, Andreas
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Drexler, Wolfgang
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Anderson, Peter E.
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Dholakia, Kishan
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3 May 2017
Piksarv, Peeter
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Marti, Dominik
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Le, Tuan
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Unterhuber, Angelika
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Forbes, Lindsey
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Andrews, Melissa R.
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Stingl, Andreas
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Drexler, Wolfgang
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Anderson, Peter E.
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Dholakia, Kishan
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Piksarv, Peeter, Marti, Dominik, Le, Tuan, Unterhuber, Angelika, Forbes, Lindsey, Andrews, Melissa R., Stingl, Andreas, Drexler, Wolfgang, Anderson, Peter E. and Dholakia, Kishan
(2017)
Integrated single- and two-photon light sheet microscopy using accelerating beams.
Scientific Reports, 7, , [1435].
(doi:10.1038/s41598-017-01543-4).
Abstract
We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
Text
Piksarv et al integrated_single_two_5
- Accepted Manuscript
Text
s41598-017-01543-4
- Version of Record
More information
Accepted/In Press date: 31 March 2017
Published date: 3 May 2017
Additional Information:
P.P. acknowledges funding by the Estonian Research Council (grant PUTJD8).
Organisations:
Biomedicine
Identifiers
Local EPrints ID: 407675
URI: http://eprints.soton.ac.uk/id/eprint/407675
PURE UUID: b98100fb-88ed-4a4c-8080-a782d1d3fb3f
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Date deposited: 22 Apr 2017 01:03
Last modified: 16 Mar 2024 05:15
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Contributors
Author:
Peeter Piksarv
Author:
Dominik Marti
Author:
Tuan Le
Author:
Angelika Unterhuber
Author:
Lindsey Forbes
Author:
Andreas Stingl
Author:
Wolfgang Drexler
Author:
Peter E. Anderson
Author:
Kishan Dholakia
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