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Pore geometry regulates early stage human bone marrow cell tissue formation and organisation

Pore geometry regulates early stage human bone marrow cell tissue formation and organisation
Pore geometry regulates early stage human bone marrow cell tissue formation and organisation
Porous architecture has a dramatic effect on tissue formation in porous biomaterials used in regenerative medicine. However, the wide variety of 3D structures used indicates there is a clear need for the optimal design of pore architecture to maximize tissue formation and ingrowth. Thus, the aim of this study was to characterize initial tissue growth solely as a function of pore geometry. We used an in vitro system with well-defined open pore slots of varying width, providing a 3D environment for neo-tissue formation while minimizing nutrient limitations. Results demonstrated that initial tissue formation was strongly influenced by pore geometry. Both velocity of tissue invasion and area of tissue formed increased as pores became narrower. This is associated with distinct patterns of actin organisation and alignment depending on pore width, indicating the role of active cell generated forces. A mathematical model based on curvature driven growth successfully predicted both shape of invasion front and constant rate of growth, which increased for narrower pores as seen in experiments. Our results provide further evidence for a front based, curvature driven growth mechanism depending on pore geometry and tissue organisation, which could provide important clues for 3D scaffold design.
Actin, Calcium phosphate cements, Human bone marrow cells, Mathematical modelling, Porous scaffolds, Tissue engineering
0090-6964
917-930
Knychala, J.
685475ea-fd0a-4458-a58b-91f661c2f438
Bouropoulos, N.
e9148b15-054b-40ba-aada-1f13575ada3c
Catt, C. J.
0733d0a3-19bd-4043-a059-00ade084d867
Katsamenis, O. L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Please, C. P.
118dffe7-4b38-4787-a972-9feec535839e
Sengers, B. G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Knychala, J.
685475ea-fd0a-4458-a58b-91f661c2f438
Bouropoulos, N.
e9148b15-054b-40ba-aada-1f13575ada3c
Catt, C. J.
0733d0a3-19bd-4043-a059-00ade084d867
Katsamenis, O. L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Please, C. P.
118dffe7-4b38-4787-a972-9feec535839e
Sengers, B. G.
d6b771b1-4ede-48c5-9644-fa86503941aa

Knychala, J., Bouropoulos, N., Catt, C. J., Katsamenis, O. L., Please, C. P. and Sengers, B. G. (2013) Pore geometry regulates early stage human bone marrow cell tissue formation and organisation. Annals of Biomedical Engineering, 41, 917-930. (doi:10.1007/s10439-013-0748-z).

Record type: Article

Abstract

Porous architecture has a dramatic effect on tissue formation in porous biomaterials used in regenerative medicine. However, the wide variety of 3D structures used indicates there is a clear need for the optimal design of pore architecture to maximize tissue formation and ingrowth. Thus, the aim of this study was to characterize initial tissue growth solely as a function of pore geometry. We used an in vitro system with well-defined open pore slots of varying width, providing a 3D environment for neo-tissue formation while minimizing nutrient limitations. Results demonstrated that initial tissue formation was strongly influenced by pore geometry. Both velocity of tissue invasion and area of tissue formed increased as pores became narrower. This is associated with distinct patterns of actin organisation and alignment depending on pore width, indicating the role of active cell generated forces. A mathematical model based on curvature driven growth successfully predicted both shape of invasion front and constant rate of growth, which increased for narrower pores as seen in experiments. Our results provide further evidence for a front based, curvature driven growth mechanism depending on pore geometry and tissue organisation, which could provide important clues for 3D scaffold design.

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More information

e-pub ahead of print date: 13 February 2013
Published date: May 2013
Keywords: Actin, Calcium phosphate cements, Human bone marrow cells, Mathematical modelling, Porous scaffolds, Tissue engineering
Organisations: Engineering Mats & Surface Engineerg Gp, Bioengineering Group, Mathematical Sciences, Mathematics, Education Hub, Engineering Sciences

Identifiers

Local EPrints ID: 407861
URI: https://eprints.soton.ac.uk/id/eprint/407861
ISSN: 0090-6964
PURE UUID: 095ade17-5695-4ad2-adf1-ae8cc9ab0f6f
ORCID for O. L. Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147
ORCID for B. G. Sengers: ORCID iD orcid.org/0000-0001-5859-6984

Catalogue record

Date deposited: 27 Apr 2017 01:08
Last modified: 14 Mar 2019 01:41

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Contributors

Author: J. Knychala
Author: N. Bouropoulos
Author: C. J. Catt
Author: C. P. Please
Author: B. G. Sengers ORCID iD

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