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PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations

PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations
PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations

AIM: To fabricate PEGylated liposomes which preserve the activity of hydrophobic Wnt3A protein, and to demonstrate their efficacy in promoting expansion of osteoprogenitors from human bone marrow.

METHODS: PEGylated liposomes composed of several synthetic lipids were tested for their ability to preserve Wnt3A activity in reporter and differentiation assays. Single-molecule microspectroscopy was used to test for direct association of protein with liposomes.

RESULTS: Labeled Wnt3A protein directly associated with all tested liposome preparations. However, Wnt3A activity was preserved or enhanced in PEGylated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes but not in PEGylated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes. PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis.

CONCLUSION: Active Wnt protein-containing PEGylated liposomes may have utility for systemic administration for bone repair.

Journal Article
1743-5889
845-863
Janeczek, Agnieszka A
6d713f66-c5fb-40f7-9a7d-17415eb66ec7
Scarpa, Edoardo
e7d094b2-e64f-4b4c-a452-d478a912b171
Horrocks, Mathew H.
aa3df2cd-16e8-45d1-95ba-e816b104f8c2
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Rowland, Caroline A.
61ff9bdd-665a-406d-90ef-bd84777f6103
Jenner, Dominic
a1941a7b-54e1-4e18-bb24-4235448c7bd3
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Lee, Steven F.
171a9b9f-a278-4bb7-b266-eeda062d05c3
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Janeczek, Agnieszka A
6d713f66-c5fb-40f7-9a7d-17415eb66ec7
Scarpa, Edoardo
e7d094b2-e64f-4b4c-a452-d478a912b171
Horrocks, Mathew H.
aa3df2cd-16e8-45d1-95ba-e816b104f8c2
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Rowland, Caroline A.
61ff9bdd-665a-406d-90ef-bd84777f6103
Jenner, Dominic
a1941a7b-54e1-4e18-bb24-4235448c7bd3
Newman, Tracey A.
322290cb-2e9c-445d-a047-00b1bea39a25
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Lee, Steven F.
171a9b9f-a278-4bb7-b266-eeda062d05c3
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95

Janeczek, Agnieszka A, Scarpa, Edoardo, Horrocks, Mathew H., Tare, Rahul S., Rowland, Caroline A., Jenner, Dominic, Newman, Tracey A., Oreffo, Richard O.C., Lee, Steven F. and Evans, Nicholas D. (2017) PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations. Nanomedicine, 12 (8), 845-863. (doi:10.2217/nnm-2016-0386).

Record type: Article

Abstract

AIM: To fabricate PEGylated liposomes which preserve the activity of hydrophobic Wnt3A protein, and to demonstrate their efficacy in promoting expansion of osteoprogenitors from human bone marrow.

METHODS: PEGylated liposomes composed of several synthetic lipids were tested for their ability to preserve Wnt3A activity in reporter and differentiation assays. Single-molecule microspectroscopy was used to test for direct association of protein with liposomes.

RESULTS: Labeled Wnt3A protein directly associated with all tested liposome preparations. However, Wnt3A activity was preserved or enhanced in PEGylated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes but not in PEGylated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes. PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis.

CONCLUSION: Active Wnt protein-containing PEGylated liposomes may have utility for systemic administration for bone repair.

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More information

Accepted/In Press date: 7 February 2017
e-pub ahead of print date: 29 March 2017
Published date: April 2017
Keywords: Journal Article
Organisations: Medicine: Faculty Office, Bioengineering Group, Human Development & Health, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 408022
URI: https://eprints.soton.ac.uk/id/eprint/408022
ISSN: 1743-5889
PURE UUID: 9ef3340c-6d81-4104-96ab-0ceb79898dfb
ORCID for Rahul S. Tare: ORCID iD orcid.org/0000-0001-8274-8837
ORCID for Tracey A. Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 09 May 2017 01:04
Last modified: 10 Dec 2019 01:54

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