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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors

Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.
0956-5663
281-287
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Kalsi, Sumit
0ec1f5c2-a6ec-4c07-92fb-15708c28742e
Zeimpekis, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
572b5f93-dfb9-495c-8e82-52a9d373b0ed
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Turner, Carrie
8ff2a882-4649-4031-a660-7b5889169557
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hu, Chunxiao
4892b566-6809-42a8-8285-1c1e93aac730
Kalsi, Sumit
0ec1f5c2-a6ec-4c07-92fb-15708c28742e
Zeimpekis, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
572b5f93-dfb9-495c-8e82-52a9d373b0ed
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Turner, Carrie
8ff2a882-4649-4031-a660-7b5889169557
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Hu, Chunxiao, Kalsi, Sumit, Zeimpekis, Ioannis, Sun, Kai, Ashburn, Peter, Turner, Carrie, Sutton, J. Mark and Morgan, Hywel (2017) Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors. Biosensors & Bioelectronics, 96, 281-287. (doi:10.1016/j.bios.2017.05.016).

Record type: Article

Abstract

A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.

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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors - Accepted Manuscript
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More information

Accepted/In Press date: 8 May 2017
e-pub ahead of print date: 9 May 2017
Published date: 15 October 2017
Organisations: Optoelectronics Research Centre, Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 408072
URI: https://eprints.soton.ac.uk/id/eprint/408072
ISSN: 0956-5663
PURE UUID: 3024968b-40d6-42fb-8c42-98abdc3f45b2
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 11 May 2017 01:03
Last modified: 10 Sep 2019 04:54

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Contributors

Author: Chunxiao Hu
Author: Sumit Kalsi
Author: Ioannis Zeimpekis
Author: Kai Sun
Author: Peter Ashburn
Author: Carrie Turner
Author: J. Mark Sutton
Author: Hywel Morgan ORCID iD

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