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Microfluidic electrochemical multiplex detection of bladder cancer DNA markers

Microfluidic electrochemical multiplex detection of bladder cancer DNA markers
Microfluidic electrochemical multiplex detection of bladder cancer DNA markers
Bladder cancer (BC) is one of the most difficult cancers to diagnose, and is reportedly the most expensive cancer to treat and monitor due to the lack of accurate chemical sensors; only limited methods are available, which usually are restricted to cytology and cystoscopy. Thus a system is urgently needed to detect biomarkers that give an unambiguous diagnosis on BC, as it currently is among the top five cancers occurring. Here we present a multi-sensor microchip array which efficiently detects three specific bladder cancer DNA markers simultaneously with a detection limit of 250 fM, which is well below the amount of DNA markers found in urine samples. The detection is based on the electrochemical response of a free-base porphyrin marker which is embedded in a molecular beacon; this system can easily be adapted to detect other DNA markers and developed for field applications, including point-of-care diagnostics, and gives an unambiguous readout within 20 minutes.
Multiplex DNA detection, bladder cancer, microfluidic microchip sensor, porphyrin labelled DNA, voltammetry
0925-4005
Chen, Yu
b7c530d5-7aa6-4515-ac71-907f6673423b
Pursey, Joanna, Patricia
f5e36098-76a3-4e39-9d32-7827879799aa
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Park, Mi Kyoung
a4713b01-4b81-40b7-b742-d48fddaf093e
Kongsuphol, Patthara
9fc87d10-cd4f-4648-883e-fa83bc5f420d
Chen, Yu
b7c530d5-7aa6-4515-ac71-907f6673423b
Pursey, Joanna, Patricia
f5e36098-76a3-4e39-9d32-7827879799aa
Stulz, Eugen
9a6c04cf-32ca-442b-9281-bbf3d23c622d
Park, Mi Kyoung
a4713b01-4b81-40b7-b742-d48fddaf093e
Kongsuphol, Patthara
9fc87d10-cd4f-4648-883e-fa83bc5f420d

Chen, Yu, Pursey, Joanna, Patricia, Stulz, Eugen, Park, Mi Kyoung and Kongsuphol, Patthara (2017) Microfluidic electrochemical multiplex detection of bladder cancer DNA markers. Sensors and Actuators B: Chemical. (doi:10.1016/j.snb.2017.05.006).

Record type: Article

Abstract

Bladder cancer (BC) is one of the most difficult cancers to diagnose, and is reportedly the most expensive cancer to treat and monitor due to the lack of accurate chemical sensors; only limited methods are available, which usually are restricted to cytology and cystoscopy. Thus a system is urgently needed to detect biomarkers that give an unambiguous diagnosis on BC, as it currently is among the top five cancers occurring. Here we present a multi-sensor microchip array which efficiently detects three specific bladder cancer DNA markers simultaneously with a detection limit of 250 fM, which is well below the amount of DNA markers found in urine samples. The detection is based on the electrochemical response of a free-base porphyrin marker which is embedded in a molecular beacon; this system can easily be adapted to detect other DNA markers and developed for field applications, including point-of-care diagnostics, and gives an unambiguous readout within 20 minutes.

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More information

Accepted/In Press date: 3 May 2017
Published date: 4 May 2017
Keywords: Multiplex DNA detection, bladder cancer, microfluidic microchip sensor, porphyrin labelled DNA, voltammetry
Organisations: CBDT, Chemistry

Identifiers

Local EPrints ID: 408109
URI: https://eprints.soton.ac.uk/id/eprint/408109
ISSN: 0925-4005
PURE UUID: f82537bc-dc7d-42f9-b289-55b84ebb5059
ORCID for Eugen Stulz: ORCID iD orcid.org/0000-0002-5302-2276

Catalogue record

Date deposited: 12 May 2017 04:03
Last modified: 14 Mar 2019 01:41

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Contributors

Author: Yu Chen
Author: Joanna, Patricia Pursey
Author: Eugen Stulz ORCID iD
Author: Mi Kyoung Park
Author: Patthara Kongsuphol

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