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Chromogranin A promotes peptide hormone sorting to mobile granules in constitutively and regulated secreting cells: ROLE OF CONSERVED N- AND C-TERMINAL PEPTIDES

Chromogranin A promotes peptide hormone sorting to mobile granules in constitutively and regulated secreting cells: ROLE OF CONSERVED N- AND C-TERMINAL PEPTIDES
Chromogranin A promotes peptide hormone sorting to mobile granules in constitutively and regulated secreting cells: ROLE OF CONSERVED N- AND C-TERMINAL PEPTIDES
Chromogranin A (CgA) has been proposed to play a major role in the formation of dense-core secretory granules (DCGs) in neuroendocrine cells. Here, we took advantage of unique features of the frog CgA (fCgA) to assess the role of this granin and its potential functional determinants in hormone sorting during DCG biogenesis. Expression of fCgA in the constitutively secreting COS-7 cells induced the formation of mobile vesicular structures, which contained cotransfected peptide hormones. The fCgA and the hormones coexpressed in the newly formed vesicles could be released in a regulated manner. The N- and C-terminal regions of fCgA, which exhibit remarkable sequence conservation with their mammalian counterparts were found to be essential for the formation of the mobile DCG-like structures in COS-7 cells. Expression of fCgA in the corticotrope AtT20 cells increased pro-opiomelanocortin levels in DCGs, whereas the expression of N- and C-terminal deletion mutants provoked retention of the hormone in the Golgi area. Furthermore, fCgA, but not its truncated forms, promoted pro-opiomelanocortin sorting to the regulated secretory pathway. These data demonstrate that CgA has the intrinsic capacity to induce the formation of mobile secretory granules and to promote the sorting and release of peptide hormones. The conserved terminal peptides are instrumental for these activities of CgA.
1083-351X
12420-12431
Maité, Montero-Hadjadje
e1fb2ba7-e4d9-4282-bea8-350ee1778526
Elias, Salah
a9b11116-8efb-44b3-8241-2f0f2af847c3
Chevalier, Laurence
469f51f2-d7dd-48d9-a81d-5513f9c2724e
Benard, Magalie
ad171be0-f72b-43fa-999f-61bb16d06bf4
Tanguy, Yannick
1ed90f89-e18e-4615-9b97-4c9ae5ff2718
Turquier, Valérie
b71ee1e5-7e3a-4eb5-b10b-2378bb8d2175
Galas, Ludovic
66a53ba3-892e-4bf5-a063-217ae3d7c1d0
Yon, Laurent
2e709dd3-949c-4d17-89e6-8ed69fbd0da3
Malagon, Maria
349f269c-6b9d-4a82-a213-5d390250fc98
Driouich, Azeddine
0dddef5f-34a4-4fc1-b365-95ce1d477a65
Gasman, Stéphane
78bca850-b59f-4b9d-b936-47a57287a3f0
Anouar, Youssef
a19c61e2-e59d-417f-981b-f88eccf492b2
Maité, Montero-Hadjadje
e1fb2ba7-e4d9-4282-bea8-350ee1778526
Elias, Salah
a9b11116-8efb-44b3-8241-2f0f2af847c3
Chevalier, Laurence
469f51f2-d7dd-48d9-a81d-5513f9c2724e
Benard, Magalie
ad171be0-f72b-43fa-999f-61bb16d06bf4
Tanguy, Yannick
1ed90f89-e18e-4615-9b97-4c9ae5ff2718
Turquier, Valérie
b71ee1e5-7e3a-4eb5-b10b-2378bb8d2175
Galas, Ludovic
66a53ba3-892e-4bf5-a063-217ae3d7c1d0
Yon, Laurent
2e709dd3-949c-4d17-89e6-8ed69fbd0da3
Malagon, Maria
349f269c-6b9d-4a82-a213-5d390250fc98
Driouich, Azeddine
0dddef5f-34a4-4fc1-b365-95ce1d477a65
Gasman, Stéphane
78bca850-b59f-4b9d-b936-47a57287a3f0
Anouar, Youssef
a19c61e2-e59d-417f-981b-f88eccf492b2

Maité, Montero-Hadjadje, Elias, Salah, Chevalier, Laurence, Benard, Magalie, Tanguy, Yannick, Turquier, Valérie, Galas, Ludovic, Yon, Laurent, Malagon, Maria, Driouich, Azeddine, Gasman, Stéphane and Anouar, Youssef (2009) Chromogranin A promotes peptide hormone sorting to mobile granules in constitutively and regulated secreting cells: ROLE OF CONSERVED N- AND C-TERMINAL PEPTIDES. The Journal of Biological Chemistry, 284, 12420-12431. (doi:10.1074/jbc.M805607200).

Record type: Article

Abstract

Chromogranin A (CgA) has been proposed to play a major role in the formation of dense-core secretory granules (DCGs) in neuroendocrine cells. Here, we took advantage of unique features of the frog CgA (fCgA) to assess the role of this granin and its potential functional determinants in hormone sorting during DCG biogenesis. Expression of fCgA in the constitutively secreting COS-7 cells induced the formation of mobile vesicular structures, which contained cotransfected peptide hormones. The fCgA and the hormones coexpressed in the newly formed vesicles could be released in a regulated manner. The N- and C-terminal regions of fCgA, which exhibit remarkable sequence conservation with their mammalian counterparts were found to be essential for the formation of the mobile DCG-like structures in COS-7 cells. Expression of fCgA in the corticotrope AtT20 cells increased pro-opiomelanocortin levels in DCGs, whereas the expression of N- and C-terminal deletion mutants provoked retention of the hormone in the Golgi area. Furthermore, fCgA, but not its truncated forms, promoted pro-opiomelanocortin sorting to the regulated secretory pathway. These data demonstrate that CgA has the intrinsic capacity to induce the formation of mobile secretory granules and to promote the sorting and release of peptide hormones. The conserved terminal peptides are instrumental for these activities of CgA.

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e-pub ahead of print date: 29 January 2009
Published date: 1 May 2009
Organisations: Biomedicine

Identifiers

Local EPrints ID: 408243
URI: https://eprints.soton.ac.uk/id/eprint/408243
ISSN: 1083-351X
PURE UUID: 3495a8de-460c-447e-97ff-d0d0361982ab

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Date deposited: 18 May 2017 04:01
Last modified: 13 Mar 2019 19:57

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Contributors

Author: Montero-Hadjadje Maité
Author: Salah Elias
Author: Laurence Chevalier
Author: Magalie Benard
Author: Yannick Tanguy
Author: Valérie Turquier
Author: Ludovic Galas
Author: Laurent Yon
Author: Maria Malagon
Author: Azeddine Driouich
Author: Stéphane Gasman
Author: Youssef Anouar

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