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Quantifying the percentage of methane formation via acetoclastic and syntrophic acetate oxidation pathways in anaerobic digesters

Quantifying the percentage of methane formation via acetoclastic and syntrophic acetate oxidation pathways in anaerobic digesters
Quantifying the percentage of methane formation via acetoclastic and syntrophic acetate oxidation pathways in anaerobic digesters
Ammonia concentration is one of the key factors influencing the methanogenic community composition and dominant methanogenic pathway in anaerobic digesters. This study adopted a radiolabelling technique using [2-14C] acetate to investigate the relationship between total ammonia nitrogen (TAN) and the methanogenic pathway. The radiolabelling experiments determined the ratio of 14CO2 and 14CH4 in the biogas which was used to quantitatively determine the percentage of CH4 derived from acetoclastic and syntrophic acetate oxidation routes, respectively.
This technique was performed on a selection of mesophilic digesters representing samples of low to high TAN concentrations (0.2-11.1 g kg-1 wet weight). In high TAN digesters, the ratio between 14CO2 and 14CH4 was in the range 2.1-3.0; indicating 68-75% of methane was produced via the hydrogenotrophic route; whereas in low ammonia samples the ratio was 0.1-0.3, indicating 9-23% of methane was produced by the hydrogenotrophic route. These findings have been confirmed further by phylogenetic studies.
0956-053X
749-756
Jiang, Ying
db0be779-4c23-4d32-8d5d-2bbca80e6247
Banks, Charles
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Zhang, Yue
69b11d32-d555-46e4-a333-88eee4628ae7
Heaven, Sonia
f25f74b6-97bd-4a18-b33b-a63084718571
Longhurst, Philip
317cc39c-5875-4685-90c4-bf725ce3212b
Jiang, Ying
db0be779-4c23-4d32-8d5d-2bbca80e6247
Banks, Charles
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Zhang, Yue
69b11d32-d555-46e4-a333-88eee4628ae7
Heaven, Sonia
f25f74b6-97bd-4a18-b33b-a63084718571
Longhurst, Philip
317cc39c-5875-4685-90c4-bf725ce3212b

Jiang, Ying, Banks, Charles, Zhang, Yue, Heaven, Sonia and Longhurst, Philip (2018) Quantifying the percentage of methane formation via acetoclastic and syntrophic acetate oxidation pathways in anaerobic digesters. Waste Management, 71, 749-756. (doi:10.1016/j.wasman.2017.04.005).

Record type: Article

Abstract

Ammonia concentration is one of the key factors influencing the methanogenic community composition and dominant methanogenic pathway in anaerobic digesters. This study adopted a radiolabelling technique using [2-14C] acetate to investigate the relationship between total ammonia nitrogen (TAN) and the methanogenic pathway. The radiolabelling experiments determined the ratio of 14CO2 and 14CH4 in the biogas which was used to quantitatively determine the percentage of CH4 derived from acetoclastic and syntrophic acetate oxidation routes, respectively.
This technique was performed on a selection of mesophilic digesters representing samples of low to high TAN concentrations (0.2-11.1 g kg-1 wet weight). In high TAN digesters, the ratio between 14CO2 and 14CH4 was in the range 2.1-3.0; indicating 68-75% of methane was produced via the hydrogenotrophic route; whereas in low ammonia samples the ratio was 0.1-0.3, indicating 9-23% of methane was produced by the hydrogenotrophic route. These findings have been confirmed further by phylogenetic studies.

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Accepted/In Press date: 2 April 2017
e-pub ahead of print date: 7 April 2017
Published date: January 2018
Organisations: Water & Environmental Engineering Group

Identifiers

Local EPrints ID: 408304
URI: https://eprints.soton.ac.uk/id/eprint/408304
ISSN: 0956-053X
PURE UUID: 7f61870a-9065-4395-9033-fe69cbe05e08
ORCID for Yue Zhang: ORCID iD orcid.org/0000-0002-5068-2260

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Date deposited: 19 May 2017 04:02
Last modified: 03 Dec 2019 01:51

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Contributors

Author: Ying Jiang
Author: Charles Banks
Author: Yue Zhang ORCID iD
Author: Sonia Heaven
Author: Philip Longhurst

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