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Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits
Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits
The eukaryotic translation initiation factor eIF2B is a multi-subunit complex with a crucial role in the regulation of global protein synthesis in the cell. The complex comprises five subunits, termed α through ε in order of increasing size, arranged as a heterodecamer with two copies of each subunit. Regulation of the co-stoichiometric expression of the eIF2B subunits is crucial for the proper function and regulation of the eIF2B complex in cells. We have investigated the control of stoichiometric eIF2B complexes through mutual stabilization of eIF2B subunits. Our data show that the stable expression of the catalytic eIF2Bε subunit in human cells requires co-expression of eIF2Bγ. Similarly, stable expression of eIF2Bδ requires both eIF2Bβ and eIF2Bγ+ε. The expression of these subunits decreases despite there being no change in either the levels or the translation of their mRNAs. Instead, these subunits are targeted for degradation by the ubiquitin-proteasome system. The data allow us to propose a model for the formation of stoichiometric eIF2B complexes which can ensure their stoichiometric incorporation into the holocomplex.
1470-8728
571-580
Wortham, Noel
9b0a219d-41fa-4f83-9621-6ffd60f44c8f
Stewart, Joanna
e1ec9784-39cc-48ed-9f4f-2a05d25f2106
Harris, Sean
39be023f-632d-49d5-9cef-45a5ca984b36
Coldwell, Mark
a3432799-ed45-4948-9f7a-2a284d3ec65c
Proud, Christopher G.
5832db56-9069-4617-a80d-08b069093dba
Wortham, Noel
9b0a219d-41fa-4f83-9621-6ffd60f44c8f
Stewart, Joanna
e1ec9784-39cc-48ed-9f4f-2a05d25f2106
Harris, Sean
39be023f-632d-49d5-9cef-45a5ca984b36
Coldwell, Mark
a3432799-ed45-4948-9f7a-2a284d3ec65c
Proud, Christopher G.
5832db56-9069-4617-a80d-08b069093dba

Wortham, Noel, Stewart, Joanna, Harris, Sean, Coldwell, Mark and Proud, Christopher G. (2016) Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits. Biochemical Journal, 473 (5), 571-580. (doi:10.1042/BJ20150828).

Record type: Article

Abstract

The eukaryotic translation initiation factor eIF2B is a multi-subunit complex with a crucial role in the regulation of global protein synthesis in the cell. The complex comprises five subunits, termed α through ε in order of increasing size, arranged as a heterodecamer with two copies of each subunit. Regulation of the co-stoichiometric expression of the eIF2B subunits is crucial for the proper function and regulation of the eIF2B complex in cells. We have investigated the control of stoichiometric eIF2B complexes through mutual stabilization of eIF2B subunits. Our data show that the stable expression of the catalytic eIF2Bε subunit in human cells requires co-expression of eIF2Bγ. Similarly, stable expression of eIF2Bδ requires both eIF2Bβ and eIF2Bγ+ε. The expression of these subunits decreases despite there being no change in either the levels or the translation of their mRNAs. Instead, these subunits are targeted for degradation by the ubiquitin-proteasome system. The data allow us to propose a model for the formation of stoichiometric eIF2B complexes which can ensure their stoichiometric incorporation into the holocomplex.

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Published date: 24 February 2016
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 408640
URI: http://eprints.soton.ac.uk/id/eprint/408640
ISSN: 1470-8728
PURE UUID: 8d68c019-124e-4011-b50f-2098cf1309a8
ORCID for Joanna Stewart: ORCID iD orcid.org/0000-0002-2608-1967
ORCID for Mark Coldwell: ORCID iD orcid.org/0000-0002-6243-3886

Catalogue record

Date deposited: 25 May 2017 04:03
Last modified: 21 Feb 2020 01:32

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Contributors

Author: Noel Wortham
Author: Joanna Stewart ORCID iD
Author: Sean Harris
Author: Mark Coldwell ORCID iD
Author: Christopher G. Proud

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