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Analysis of parasitic protozoa at the single-cell level using microfluidic impedance cytometry

Analysis of parasitic protozoa at the single-cell level using microfluidic impedance cytometry
Analysis of parasitic protozoa at the single-cell level using microfluidic impedance cytometry
At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.
2045-2322
1-11
McGrath, J.S.
fa2a112f-208f-48eb-aa6a-715b6c311134
Honrado, C.
7d6e0aa5-1ab5-4e54-8595-3acd6050f93b
Spencer, D.
4affe9f6-353a-4507-8066-0180b8dc9eaf
Horton, B.
0a7b2c92-ac74-4bc4-854a-3121dcf56871
Bridle, H.L.
39c93f77-57ac-418f-89d8-ddc9035ebfd3
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
McGrath, J.S.
fa2a112f-208f-48eb-aa6a-715b6c311134
Honrado, C.
7d6e0aa5-1ab5-4e54-8595-3acd6050f93b
Spencer, D.
4affe9f6-353a-4507-8066-0180b8dc9eaf
Horton, B.
0a7b2c92-ac74-4bc4-854a-3121dcf56871
Bridle, H.L.
39c93f77-57ac-418f-89d8-ddc9035ebfd3
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174

McGrath, J.S., Honrado, C., Spencer, D., Horton, B., Bridle, H.L. and Morgan, H. (2017) Analysis of parasitic protozoa at the single-cell level using microfluidic impedance cytometry. Scientific Reports, 7 (1), 1-11. (doi:10.1038/s41598-017-02715-y).

Record type: Article

Abstract

At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.

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More information

Accepted/In Press date: 18 April 2017
e-pub ahead of print date: 1 July 2017
Published date: 2017
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 409741
URI: https://eprints.soton.ac.uk/id/eprint/409741
ISSN: 2045-2322
PURE UUID: 5f739042-6195-4ebd-ad1f-5807d09fc790
ORCID for H. Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 01 Jun 2017 04:07
Last modified: 12 Nov 2019 06:04

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