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Enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms

Burmolle, Mette, Webb, Jeremy S., Rao, Dhana, Hansen, Lars H., Sorensen, Soren J. and Kjelleberg, Staffan (2006) Enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms Applied and Environmental Microbiology, 72, (6), pp. 3916-3923. (doi:10.1128/AEM.03022-05).

Record type: Article


Most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. However, few reports specifically address interactions within multispecies biofilms. In this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga Ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. Four isolates, Microbacterium phyllosphaerae, Shewanella japonica, Dokdonia donghaensis, and Acinetobacter lwoffii, were found to interact synergistically in biofilms formed in 96-well microtiter plates: biofilm biomass was observed to increase by >167% in biofilms formed by the four strains compared to biofilms composed of single strains. When exposed to the antibacterial agent hydrogen peroxide or tetracycline, the relative activity (exposed versus nonexposed biofilms) of the four-species biofilm was markedly higher than that in any of the single-species biofilms. Moreover, in biofilms established on glass surfaces in flow cells and subjected to invasion by the antibacterial protein-producing Pseudoalteromonas tunicata, the four-species biofilms resisted invasion to a greater extent than did the biofilms formed by the single species. Replacement of each strain by its cell-free culture supernatant suggested that synergy was dependent both on species-specific physical interactions between cells and on extracellular secreted factors or less specific interactions. In summary, our data strongly indicate that synergistic effects promote biofilm biomass and resistance of the biofilm to antimicrobial agents and bacterial invasion in multispecies biofilms.

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Published date: June 2006


Local EPrints ID: 41045
ISSN: 0099-2240
PURE UUID: 5ce7c3cd-df50-4154-b095-cd43addcd5d8
ORCID for Jeremy S. Webb: ORCID iD

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Date deposited: 14 Jul 2006
Last modified: 17 Jul 2017 15:32

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Author: Mette Burmolle
Author: Jeremy S. Webb ORCID iD
Author: Dhana Rao
Author: Lars H. Hansen
Author: Soren J. Sorensen
Author: Staffan Kjelleberg

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