The University of Southampton
University of Southampton Institutional Repository

Enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms

Record type: Article

Most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. However, few reports specifically address interactions within multispecies biofilms. In this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga Ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. Four isolates, Microbacterium phyllosphaerae, Shewanella japonica, Dokdonia donghaensis, and Acinetobacter lwoffii, were found to interact synergistically in biofilms formed in 96-well microtiter plates: biofilm biomass was observed to increase by >167% in biofilms formed by the four strains compared to biofilms composed of single strains. When exposed to the antibacterial agent hydrogen peroxide or tetracycline, the relative activity (exposed versus nonexposed biofilms) of the four-species biofilm was markedly higher than that in any of the single-species biofilms. Moreover, in biofilms established on glass surfaces in flow cells and subjected to invasion by the antibacterial protein-producing Pseudoalteromonas tunicata, the four-species biofilms resisted invasion to a greater extent than did the biofilms formed by the single species. Replacement of each strain by its cell-free culture supernatant suggested that synergy was dependent both on species-specific physical interactions between cells and on extracellular secreted factors or less specific interactions. In summary, our data strongly indicate that synergistic effects promote biofilm biomass and resistance of the biofilm to antimicrobial agents and bacterial invasion in multispecies biofilms.

PDF Burmolle_et_al_06.pdf - Other
Restricted to Repository staff only
Download (167kB)

Citation

Burmolle, Mette, Webb, Jeremy S., Rao, Dhana, Hansen, Lars H., Sorensen, Soren J. and Kjelleberg, Staffan (2006) Enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms Applied and Environmental Microbiology, 72, (6), pp. 3916-3923. (doi:10.1128/AEM.03022-05).

More information

Published date: June 2006

Identifiers

Local EPrints ID: 41045
URI: http://eprints.soton.ac.uk/id/eprint/41045
ISSN: 0099-2240
PURE UUID: 5ce7c3cd-df50-4154-b095-cd43addcd5d8
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

Catalogue record

Date deposited: 14 Jul 2006
Last modified: 17 Jul 2017 15:32

Export record

Altmetrics

Contributors

Author: Mette Burmolle
Author: Jeremy S. Webb ORCID iD
Author: Dhana Rao
Author: Lars H. Hansen
Author: Soren J. Sorensen
Author: Staffan Kjelleberg

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×