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The transcriptome of Populus in elevated CO2

The transcriptome of Populus in elevated CO2
The transcriptome of Populus in elevated CO2
The consequences of increasing atmospheric carbon dioxide for long-term adaptation of forest ecosystems remain uncertain, with virtually no studies undertaken at the genetic level. A global analysis using cDNA microarrays was conducted following 6 yr exposure of Populus × euramericana (clone I-214) to elevated [CO2] in a FACE (free-air CO2 enrichment) experiment.
Gene expression was sensitive to elevated [CO2] but the response depended on the developmental age of the leaves, and < 50 transcripts differed significantly between different CO2 environments. For young leaves most differentially expressed genes were upregulated in elevated [CO2], while in semimature leaves most were downregulated in elevated [CO2].
For transcripts related only to the small subunit of Rubisco, upregulation in LPI 3 and downregulation in LPI 6 leaves in elevated CO2 was confirmed by anova. Similar patterns of gene expression for young leaves were also confirmed independently across year 3 and year 6 microarray data, and using real-time RT–PCR.
This study provides the first clues to the long-term genetic expression changes that may occur during long-term plant response to elevated CO2.
0028-646X
143-154
Taylor, Gail
f3851db9-d37c-4c36-8663-e5c2cb03e171
Street, Nathaniel R.
059730be-153a-4108-a5b1-7fb747bd75f9
Tricker, Penny J.
a4db1985-2cd5-4356-b932-77ae8ed7d25c
Sjodin, Andreas
3609694d-9018-4d2e-8954-aefe024b5dd8
Graham, Laura
06c28e85-8429-4297-8121-2d89e458a03f
Skogstrom, Oskar
4ed3dd2b-74fd-44b9-984f-edbd1d2a6b01
Calfapietra, Carlo
de5824b5-df60-4f91-994e-1b6e2c5585b6
Scarascia-Mugnozza, Giuseppe
45a5cfda-4d26-4858-a028-0e040268e39a
Jansson, Stefan
1796ac57-d64d-43dd-97b7-7700ce349c27
Taylor, Gail
f3851db9-d37c-4c36-8663-e5c2cb03e171
Street, Nathaniel R.
059730be-153a-4108-a5b1-7fb747bd75f9
Tricker, Penny J.
a4db1985-2cd5-4356-b932-77ae8ed7d25c
Sjodin, Andreas
3609694d-9018-4d2e-8954-aefe024b5dd8
Graham, Laura
06c28e85-8429-4297-8121-2d89e458a03f
Skogstrom, Oskar
4ed3dd2b-74fd-44b9-984f-edbd1d2a6b01
Calfapietra, Carlo
de5824b5-df60-4f91-994e-1b6e2c5585b6
Scarascia-Mugnozza, Giuseppe
45a5cfda-4d26-4858-a028-0e040268e39a
Jansson, Stefan
1796ac57-d64d-43dd-97b7-7700ce349c27

Taylor, Gail, Street, Nathaniel R., Tricker, Penny J., Sjodin, Andreas, Graham, Laura, Skogstrom, Oskar, Calfapietra, Carlo, Scarascia-Mugnozza, Giuseppe and Jansson, Stefan (2005) The transcriptome of Populus in elevated CO2. New Phytologist, 167 (1), 143-154. (doi:10.1111/j.1469-8137.2005.01450.x).

Record type: Article

Abstract

The consequences of increasing atmospheric carbon dioxide for long-term adaptation of forest ecosystems remain uncertain, with virtually no studies undertaken at the genetic level. A global analysis using cDNA microarrays was conducted following 6 yr exposure of Populus × euramericana (clone I-214) to elevated [CO2] in a FACE (free-air CO2 enrichment) experiment.
Gene expression was sensitive to elevated [CO2] but the response depended on the developmental age of the leaves, and < 50 transcripts differed significantly between different CO2 environments. For young leaves most differentially expressed genes were upregulated in elevated [CO2], while in semimature leaves most were downregulated in elevated [CO2].
For transcripts related only to the small subunit of Rubisco, upregulation in LPI 3 and downregulation in LPI 6 leaves in elevated CO2 was confirmed by anova. Similar patterns of gene expression for young leaves were also confirmed independently across year 3 and year 6 microarray data, and using real-time RT–PCR.
This study provides the first clues to the long-term genetic expression changes that may occur during long-term plant response to elevated CO2.

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Published date: 2005

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Local EPrints ID: 41066
URI: https://eprints.soton.ac.uk/id/eprint/41066
ISSN: 0028-646X
PURE UUID: e1065023-68a3-408b-87cc-2b5e46ac5c7a

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Date deposited: 14 Jul 2006
Last modified: 17 Jul 2017 15:32

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Contributors

Author: Gail Taylor
Author: Nathaniel R. Street
Author: Penny J. Tricker
Author: Andreas Sjodin
Author: Laura Graham
Author: Oskar Skogstrom
Author: Carlo Calfapietra
Author: Giuseppe Scarascia-Mugnozza
Author: Stefan Jansson

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