The University of Southampton
University of Southampton Institutional Repository

Animals lacking link protein have attenuated perineuronal nets and persistent plasticity

Animals lacking link protein have attenuated perineuronal nets and persistent plasticity
Animals lacking link protein have attenuated perineuronal nets and persistent plasticity

Chondroitin sulphate proteoglycans in the extracellular matrix restrict plasticity in the adult central nervous system and their digestion with chondroitinase reactivates plasticity. However the structures in the extracellular matrix that restrict plasticity are unknown. There are many changes in the extracellular matrix as critical periods for plasticity close, including changes in chondroitin sulphate proteoglycan core protein levels, changes in glycosaminoglycan sulphation and the appearance of dense chondroitin sulphate proteoglycan-containing perineuronal nets around many neurons. We show that formation of perineuronal nets is triggered by neuronal production of cartilage link protein Crtl1 (Hapln1), which is up-regulated in the visual cortex as perineuronal nets form during development and after dark rearing. Mice lacking Crtl1 have attenuated perineuronal nets, but the overall levels of chondroitin sulphate proteoglycans and their pattern of glycan sulphation are unchanged. Crtl1 knockout animals retain juvenile levels of ocular dominance plasticity and their visual acuity remains sensitive to visual deprivation. In the sensory pathway, axons in knockout animals but not controls sprout into the party denervated cuneate nucleus. The organization of chondroitin sulphate proteoglycan into perineuronal nets is therefore the key event in the control of central nervous system plasticity by the extracellular matrix.

extracellular matrix, plasticity, ocular dominance, proteoglycan, GABA, interneurons, synapse, link protein
0006-8950
2331-2347
Carulli, Daniela
a89141f0-e558-4b34-be99-829651ff4f4c
Pizzorusso, Tommaso
fd491a61-8eab-4f78-82a3-4f26faab200e
Kwok, Jessica C.F.
009d0931-72ad-4978-963e-e9dce272644a
Putignano, Elena
69836984-e29a-4ed8-b6b4-f4f9e448db2f
Poli, Andrea
b34b928d-f5c2-4544-b969-201cb5bbedfc
Forostyak, Serhiy
ba172435-d61b-44f0-925d-647ee64e6479
Andrews, Melissa R.
ae987a2f-878e-4ae3-a7a3-a7170712096c
Deepa, Sathyaseelan S.
9b8b05d5-6a09-4d28-b2a4-3b3172b186b9
Glant, Tibor T.
af553cb4-8a92-4a74-a601-956dde8aec8c
Fawcett, James W.
4549730e-9f62-45b8-820b-8a9c98d1058b
Carulli, Daniela
a89141f0-e558-4b34-be99-829651ff4f4c
Pizzorusso, Tommaso
fd491a61-8eab-4f78-82a3-4f26faab200e
Kwok, Jessica C.F.
009d0931-72ad-4978-963e-e9dce272644a
Putignano, Elena
69836984-e29a-4ed8-b6b4-f4f9e448db2f
Poli, Andrea
b34b928d-f5c2-4544-b969-201cb5bbedfc
Forostyak, Serhiy
ba172435-d61b-44f0-925d-647ee64e6479
Andrews, Melissa R.
ae987a2f-878e-4ae3-a7a3-a7170712096c
Deepa, Sathyaseelan S.
9b8b05d5-6a09-4d28-b2a4-3b3172b186b9
Glant, Tibor T.
af553cb4-8a92-4a74-a601-956dde8aec8c
Fawcett, James W.
4549730e-9f62-45b8-820b-8a9c98d1058b

Carulli, Daniela, Pizzorusso, Tommaso, Kwok, Jessica C.F., Putignano, Elena, Poli, Andrea, Forostyak, Serhiy, Andrews, Melissa R., Deepa, Sathyaseelan S., Glant, Tibor T. and Fawcett, James W. (2010) Animals lacking link protein have attenuated perineuronal nets and persistent plasticity. Brain, 133 (8), 2331-2347. (doi:10.1093/brain/awq145).

Record type: Article

Abstract

Chondroitin sulphate proteoglycans in the extracellular matrix restrict plasticity in the adult central nervous system and their digestion with chondroitinase reactivates plasticity. However the structures in the extracellular matrix that restrict plasticity are unknown. There are many changes in the extracellular matrix as critical periods for plasticity close, including changes in chondroitin sulphate proteoglycan core protein levels, changes in glycosaminoglycan sulphation and the appearance of dense chondroitin sulphate proteoglycan-containing perineuronal nets around many neurons. We show that formation of perineuronal nets is triggered by neuronal production of cartilage link protein Crtl1 (Hapln1), which is up-regulated in the visual cortex as perineuronal nets form during development and after dark rearing. Mice lacking Crtl1 have attenuated perineuronal nets, but the overall levels of chondroitin sulphate proteoglycans and their pattern of glycan sulphation are unchanged. Crtl1 knockout animals retain juvenile levels of ocular dominance plasticity and their visual acuity remains sensitive to visual deprivation. In the sensory pathway, axons in knockout animals but not controls sprout into the party denervated cuneate nucleus. The organization of chondroitin sulphate proteoglycan into perineuronal nets is therefore the key event in the control of central nervous system plasticity by the extracellular matrix.

Full text not available from this repository.

More information

Accepted/In Press date: 29 April 2010
e-pub ahead of print date: 20 June 2010
Published date: August 2010
Keywords: extracellular matrix, plasticity, ocular dominance, proteoglycan, GABA, interneurons, synapse, link protein
Organisations: Biomedicine

Identifiers

Local EPrints ID: 411366
URI: http://eprints.soton.ac.uk/id/eprint/411366
ISSN: 0006-8950
PURE UUID: 11a287b1-0e90-4206-aad5-a798c8a7feb1
ORCID for Melissa R. Andrews: ORCID iD orcid.org/0000-0001-5960-5619

Catalogue record

Date deposited: 19 Jun 2017 16:31
Last modified: 27 Jan 2020 13:51

Export record

Altmetrics

Contributors

Author: Daniela Carulli
Author: Tommaso Pizzorusso
Author: Jessica C.F. Kwok
Author: Elena Putignano
Author: Andrea Poli
Author: Serhiy Forostyak
Author: Sathyaseelan S. Deepa
Author: Tibor T. Glant
Author: James W. Fawcett

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×