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Circulating granulocytic and erythroid progenitor cells in chronic granulocytic leukaemia

Circulating granulocytic and erythroid progenitor cells in chronic granulocytic leukaemia
Circulating granulocytic and erythroid progenitor cells in chronic granulocytic leukaemia

We used a standard methyl cellulose method to assay erythroid progenitor cells in the blood of 35 patients with untreated CGL and of 18 normal controls. In 28 patients we simultaneously assayed granulocyte/monocyte committed progenitor cells (CFU-c) by an agar method. Circulating erythroid burst-forming units (BFU-e) in CGL were increased above normal by a factor of about 180; CFU-c were increased by a factor of about 9000. Both BFU-e and CFU-c numbers were linearly related to the total leucocyte count in individual patients but not to numbers of circulating blast cells. There was a positive correlation in individual patients between CFU-c and BFU-e numbers. Circulating BFU-e and erythroid colony-forming cells (CFU-e) were unable to proliferate in vitro in the absence of erythropoietin. We conclude that erythroid progenitor cells are involved in the 'clonal expansion' that characterizes CGL, but apparently to a lesser extent than are granulocyte/moonocyte progenitor cells.

Adolescent, Adult, Aged, Child, Colony-Forming Units Assay, Erythrocytes, Female, Granulocytes, Hematopoietic Stem Cells, Humans, Leukemia, Myeloid, Leukocyte Count, Male, Middle Aged, Journal Article, Research Support, Non-U.S. Gov't
0007-1048
7-13
Goldman, J M
2c27a1b3-c445-4593-a6b5-2e6e749fa1cb
Shiota, F
299651f1-a94a-4b2b-8778-4fabb9a94623
Th'ng, K H
e7b00688-308a-4d35-ad1d-931a409c9b1f
Orchard, K H
794654ab-d6cc-488a-ac11-c9217433c7a2
Goldman, J M
2c27a1b3-c445-4593-a6b5-2e6e749fa1cb
Shiota, F
299651f1-a94a-4b2b-8778-4fabb9a94623
Th'ng, K H
e7b00688-308a-4d35-ad1d-931a409c9b1f
Orchard, K H
794654ab-d6cc-488a-ac11-c9217433c7a2

Goldman, J M, Shiota, F, Th'ng, K H and Orchard, K H (1980) Circulating granulocytic and erythroid progenitor cells in chronic granulocytic leukaemia. British Journal of Haematology, 46 (1), 7-13.

Record type: Article

Abstract

We used a standard methyl cellulose method to assay erythroid progenitor cells in the blood of 35 patients with untreated CGL and of 18 normal controls. In 28 patients we simultaneously assayed granulocyte/monocyte committed progenitor cells (CFU-c) by an agar method. Circulating erythroid burst-forming units (BFU-e) in CGL were increased above normal by a factor of about 180; CFU-c were increased by a factor of about 9000. Both BFU-e and CFU-c numbers were linearly related to the total leucocyte count in individual patients but not to numbers of circulating blast cells. There was a positive correlation in individual patients between CFU-c and BFU-e numbers. Circulating BFU-e and erythroid colony-forming cells (CFU-e) were unable to proliferate in vitro in the absence of erythropoietin. We conclude that erythroid progenitor cells are involved in the 'clonal expansion' that characterizes CGL, but apparently to a lesser extent than are granulocyte/moonocyte progenitor cells.

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More information

Published date: September 1980
Keywords: Adolescent, Adult, Aged, Child, Colony-Forming Units Assay, Erythrocytes, Female, Granulocytes, Hematopoietic Stem Cells, Humans, Leukemia, Myeloid, Leukocyte Count, Male, Middle Aged, Journal Article, Research Support, Non-U.S. Gov't
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 411591
URI: http://eprints.soton.ac.uk/id/eprint/411591
ISSN: 0007-1048
PURE UUID: 5dea74cb-a156-4f7e-b733-a931f26e2450
ORCID for K H Orchard: ORCID iD orcid.org/0000-0003-2276-3925

Catalogue record

Date deposited: 21 Jun 2017 16:31
Last modified: 20 Jan 2024 02:40

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Contributors

Author: J M Goldman
Author: F Shiota
Author: K H Th'ng
Author: K H Orchard ORCID iD

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