A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat
A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat
Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.
Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
3234-9
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
3c2fa1fe-01a7-428d-a042-248c467c1580
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd
July 1990
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
3c2fa1fe-01a7-428d-a042-248c467c1580
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd
Orchard, K, Perkins, N, Chapman, Caroline, Harris, J, Emery, V, Goodwin, G, Latchman, D and Collins, M
(1990)
A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat.
Journal of Virology, 64 (7), .
Abstract
Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.
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More information
Published date: July 1990
Keywords:
Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
Organisations:
Electronics & Computer Science, Support Staff
Identifiers
Local EPrints ID: 411594
URI: http://eprints.soton.ac.uk/id/eprint/411594
ISSN: 0022-538X
PURE UUID: b1ed8cb1-1c43-447e-8e54-86d2e4d1706b
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Date deposited: 21 Jun 2017 16:31
Last modified: 29 Jul 2022 01:39
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Contributors
Author:
K Orchard
Author:
N Perkins
Author:
Caroline Chapman
Author:
J Harris
Author:
V Emery
Author:
G Goodwin
Author:
D Latchman
Author:
M Collins
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