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A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat

A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat
A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat

Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.

Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
0022-538X
3234-9
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
c9962744-8ebe-4837-a80a-d7290c78330b
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
c9962744-8ebe-4837-a80a-d7290c78330b
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd

Orchard, K, Perkins, N, Chapman, Caroline, Harris, J, Emery, V, Goodwin, G, Latchman, D and Collins, M (1990) A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat. Journal of Virology, 64 (7), 3234-9.

Record type: Article

Abstract

Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.

Full text not available from this repository.

More information

Published date: July 1990
Keywords: Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
Organisations: Electronics & Computer Science, Support Staff

Identifiers

Local EPrints ID: 411594
URI: https://eprints.soton.ac.uk/id/eprint/411594
ISSN: 0022-538X
PURE UUID: b1ed8cb1-1c43-447e-8e54-86d2e4d1706b
ORCID for K Orchard: ORCID iD orcid.org/0000-0003-2276-3925

Catalogue record

Date deposited: 21 Jun 2017 16:31
Last modified: 14 Mar 2019 01:45

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Contributors

Author: K Orchard ORCID iD
Author: N Perkins
Author: Caroline Chapman
Author: J Harris
Author: V Emery
Author: G Goodwin
Author: D Latchman
Author: M Collins

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