The University of Southampton
University of Southampton Institutional Repository

A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat

A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat
A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat

Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.

Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
0022-538X
3234-9
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
3c2fa1fe-01a7-428d-a042-248c467c1580
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Perkins, N
cd0fd1b8-1a84-45d3-aef1-6cee63249309
Chapman, Caroline
5292c960-8a93-4c28-a93f-2d26eda050c5
Harris, J
3c2fa1fe-01a7-428d-a042-248c467c1580
Emery, V
b2cf9949-78a6-42b8-a919-b61a985e39db
Goodwin, G
b8b6eee4-f01c-4a14-a0ef-32d5d0c94d8d
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Collins, M
39c341a9-3dab-40e3-9d5a-b77cdb360ffd

Orchard, K, Perkins, N, Chapman, Caroline, Harris, J, Emery, V, Goodwin, G, Latchman, D and Collins, M (1990) A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat. Journal of Virology, 64 (7), 3234-9.

Record type: Article

Abstract

Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.

This record has no associated files available for download.

More information

Published date: July 1990
Keywords: Base Sequence, Cell Line, DNA Mutational Analysis, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV-1, Humans, In Vitro Techniques, Methylation, Molecular Sequence Data, Nuclear Proteins, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
Organisations: Electronics & Computer Science, Support Staff

Identifiers

Local EPrints ID: 411594
URI: http://eprints.soton.ac.uk/id/eprint/411594
ISSN: 0022-538X
PURE UUID: b1ed8cb1-1c43-447e-8e54-86d2e4d1706b
ORCID for K Orchard: ORCID iD orcid.org/0000-0003-2276-3925

Catalogue record

Date deposited: 21 Jun 2017 16:31
Last modified: 29 Jul 2022 01:39

Export record

Contributors

Author: K Orchard ORCID iD
Author: N Perkins
Author: Caroline Chapman
Author: J Harris
Author: V Emery
Author: G Goodwin
Author: D Latchman
Author: M Collins

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×