Development of an ELISA for the Pharmacokinetic Evaluation of a Murine Anti CD66 Monoclonal Antibody in Human Serum
Development of an ELISA for the Pharmacokinetic Evaluation of a Murine Anti CD66 Monoclonal Antibody in Human Serum
An enzyme-linked immunosorbent assay (ELISA) was needed to assist in the pharmacokinetic evaluation of the murine antibody conjugate CHX A" DTPA Besilesomab in serum samples in a clinical trial . A search failed to locate a validated assay that quantified murine antibodies in human serum so the purpose of this article was to develop a robust assay, validated against current guidelines. A detailed method for an ELISA to measure a murine antibody in human serum is described. The assay was validated as fit for purpose against the target values of coefficient of variation < 20% and accuracy ± 20%.
Animals, Antibodies, Monoclonal, Murine-Derived, Antigens, CD, Cell Adhesion Molecules, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Plasma, Radiopharmaceuticals, Journal Article, Research Support, Non-U.S. Gov't
579-96
Langford, J
f24ea6be-95e0-4250-a698-3b134f2dcb4a
Chowdhury, F
928d9123-aed8-4ea5-87a6-297c5fe04742
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
2015
Langford, J
f24ea6be-95e0-4250-a698-3b134f2dcb4a
Chowdhury, F
928d9123-aed8-4ea5-87a6-297c5fe04742
Orchard, K
794654ab-d6cc-488a-ac11-c9217433c7a2
Langford, J, Chowdhury, F and Orchard, K
(2015)
Development of an ELISA for the Pharmacokinetic Evaluation of a Murine Anti CD66 Monoclonal Antibody in Human Serum.
Journal of Immunoassay & Immunochemistry, 36 (6), .
(doi:10.1080/15321819.2015.1017107).
Abstract
An enzyme-linked immunosorbent assay (ELISA) was needed to assist in the pharmacokinetic evaluation of the murine antibody conjugate CHX A" DTPA Besilesomab in serum samples in a clinical trial . A search failed to locate a validated assay that quantified murine antibodies in human serum so the purpose of this article was to develop a robust assay, validated against current guidelines. A detailed method for an ELISA to measure a murine antibody in human serum is described. The assay was validated as fit for purpose against the target values of coefficient of variation < 20% and accuracy ± 20%.
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Published date: 2015
Keywords:
Animals, Antibodies, Monoclonal, Murine-Derived, Antigens, CD, Cell Adhesion Molecules, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Plasma, Radiopharmaceuticals, Journal Article, Research Support, Non-U.S. Gov't
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 411605
URI: http://eprints.soton.ac.uk/id/eprint/411605
ISSN: 1532-1819
PURE UUID: d9cfe9c2-3d90-4bd6-8460-044256850371
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Date deposited: 21 Jun 2017 16:31
Last modified: 16 Mar 2024 03:26
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Author:
J Langford
Author:
F Chowdhury
Author:
K Orchard
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