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The application of silver nano-particles on developing potential treatment for chronic rhinosinusitis: Antibacterial action and cytotoxicity effect on human nasal epithelial cell model

The application of silver nano-particles on developing potential treatment for chronic rhinosinusitis: Antibacterial action and cytotoxicity effect on human nasal epithelial cell model
The application of silver nano-particles on developing potential treatment for chronic rhinosinusitis: Antibacterial action and cytotoxicity effect on human nasal epithelial cell model
Chronic rhinosinusitis (CRS) has raised attentions both in many countries due to its high relapsing rate and the resistance of certain treatments especially antibiotics application on both acute and chronic bacterial rhinosinusitis. The aim of this research is stressing on developing an effective alternative treatment for treating CRS and reducing the use of antibiotics to avoid further resistance forming. The antibacterial functions of silver nano-particles (AgNPs) are well known according to previous reports and studies. However, for developing a suitable treatment for further clinical application, a variety of AgNPs cell cytotoxicity experiments and AgNPs antibacterial properties experiments were examined in vitro in this study. For imitating the clinical condition of CRS, the human nasal epithelial cell line (RPMI2650) has chosen as experimental model. Moreover, Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) were selected for antibacterial function experiments. The analytical results demonstrated that 5 ppm of AgNPs not only maintains > 80% of cell activity to RPMI2650, but also possesses > 80% of antibacterial function to S. aureus and 100% of antibacterial function to E. coli. Therefore, 5 ppm of AgNPs might be considered as a promising antibacterial agent for treating CRS.
Chronic rhinosinusitis; Silver nano-particles; Human nasal epithelial cell;
0928-4931
Chen, Shyuan-Yow
7afd13f7-bdf5-4c3a-ac9c-49ae69044901
Huang, Min-Tsan
b37b246b-484d-4b7b-8096-5eed554107b5
Pender, Sylvia L.F.
62528b03-ec42-41bb-80fe-48454c2c5242
Ruslin, Muhammad
c930babe-8a56-45e2-a0a9-5aea1f053ff9
Chou, Hsin-Hua
2485599b-eda5-482c-9979-4f30d81d7d72
Ou, Keng-Liang
38aa7df8-00d2-441f-a898-5a5d3eb055f9
Chen, Shyuan-Yow
7afd13f7-bdf5-4c3a-ac9c-49ae69044901
Huang, Min-Tsan
b37b246b-484d-4b7b-8096-5eed554107b5
Pender, Sylvia L.F.
62528b03-ec42-41bb-80fe-48454c2c5242
Ruslin, Muhammad
c930babe-8a56-45e2-a0a9-5aea1f053ff9
Chou, Hsin-Hua
2485599b-eda5-482c-9979-4f30d81d7d72
Ou, Keng-Liang
38aa7df8-00d2-441f-a898-5a5d3eb055f9

Chen, Shyuan-Yow, Huang, Min-Tsan, Pender, Sylvia L.F., Ruslin, Muhammad, Chou, Hsin-Hua and Ou, Keng-Liang (2017) The application of silver nano-particles on developing potential treatment for chronic rhinosinusitis: Antibacterial action and cytotoxicity effect on human nasal epithelial cell model Materials Science and Engineering C (doi:10.1016/j.msec.2017.03.189).

Record type: Article

Abstract

Chronic rhinosinusitis (CRS) has raised attentions both in many countries due to its high relapsing rate and the resistance of certain treatments especially antibiotics application on both acute and chronic bacterial rhinosinusitis. The aim of this research is stressing on developing an effective alternative treatment for treating CRS and reducing the use of antibiotics to avoid further resistance forming. The antibacterial functions of silver nano-particles (AgNPs) are well known according to previous reports and studies. However, for developing a suitable treatment for further clinical application, a variety of AgNPs cell cytotoxicity experiments and AgNPs antibacterial properties experiments were examined in vitro in this study. For imitating the clinical condition of CRS, the human nasal epithelial cell line (RPMI2650) has chosen as experimental model. Moreover, Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) were selected for antibacterial function experiments. The analytical results demonstrated that 5 ppm of AgNPs not only maintains > 80% of cell activity to RPMI2650, but also possesses > 80% of antibacterial function to S. aureus and 100% of antibacterial function to E. coli. Therefore, 5 ppm of AgNPs might be considered as a promising antibacterial agent for treating CRS.

Text accepted manuscript - Accepted Manuscript
Restricted to Repository staff only until 22 March 2018.

More information

Accepted/In Press date: 21 March 2017
e-pub ahead of print date: 22 March 2017
Keywords: Chronic rhinosinusitis; Silver nano-particles; Human nasal epithelial cell;
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 411985
URI: http://eprints.soton.ac.uk/id/eprint/411985
ISSN: 0928-4931
PURE UUID: a9b56660-3946-4e82-9ca3-2a8a4fec1886
ORCID for Sylvia L.F. Pender: ORCID iD orcid.org/0000-0001-6332-0333

Catalogue record

Date deposited: 04 Jul 2017 16:31
Last modified: 02 Oct 2017 16:30

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Contributors

Author: Shyuan-Yow Chen
Author: Min-Tsan Huang
Author: Muhammad Ruslin
Author: Hsin-Hua Chou
Author: Keng-Liang Ou

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