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Serial block-face scanning electron microscopy of erythrocytes protruding through the placental syncytiotrophoblast

Serial block-face scanning electron microscopy of erythrocytes protruding through the placental syncytiotrophoblast
Serial block-face scanning electron microscopy of erythrocytes protruding through the placental syncytiotrophoblast
The syncytiotrophoblast forms a continuous barrier between the maternal and fetal circulations. Here we present a serial block-face scanning electron microscopy (SBFSEM) study, based on a single image stack, showing pooling of fetal blood underneath a region of stretched syncytiotrophoblast that has become detached from the basement membrane. Erythrocytes are protruding from discrete holes in the syncytiotrophoblast suggesting that, under specific circumstances, the syncytiotrophoblast may be permeable to fetal cells. This observation represents a pathological process but it poses questions about the physical properties and permeability of the syncytiotrophoblast and may represent an early stage in the formation of fibrin deposits in areas of syncytial denudation. This study also illustrates how the 3D images generated by SBFSEM allow the interpretation of structures that could not be understood from a single histological section.
0021-8782
Palaiologou, Eleni
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Goggin, Patricia, Mary
c2d225c4-b84d-4ead-a433-320057cb5fa9
Chatelet, David
6371fd7a-e274-4738-9ccb-3dd4dab32928
Lofthouse, Emma
c4004ff1-2ed3-4b80-9ade-583c742de59c
Torrens, Christopher
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Sengers, Bram
d6b771b1-4ede-48c5-9644-fa86503941aa
Cleal, Jane
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Page, Anton
76ebbfb8-4fe3-495c-afff-1f2f34977fee
Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Palaiologou, Eleni
a1c591e4-6bc8-4859-93af-c2d0bb9101a6
Goggin, Patricia, Mary
c2d225c4-b84d-4ead-a433-320057cb5fa9
Chatelet, David
6371fd7a-e274-4738-9ccb-3dd4dab32928
Lofthouse, Emma
c4004ff1-2ed3-4b80-9ade-583c742de59c
Torrens, Christopher
15a35713-0651-4249-8227-5901e2cfcd22
Sengers, Bram
d6b771b1-4ede-48c5-9644-fa86503941aa
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
Page, Anton
76ebbfb8-4fe3-495c-afff-1f2f34977fee
Lewis, Rohan
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502

Palaiologou, Eleni, Goggin, Patricia, Mary, Chatelet, David, Lofthouse, Emma, Torrens, Christopher, Sengers, Bram, Cleal, Jane, Page, Anton and Lewis, Rohan (2017) Serial block-face scanning electron microscopy of erythrocytes protruding through the placental syncytiotrophoblast. Journal of Anatomy. (doi:10.1111/joa.12658).

Record type: Article

Abstract

The syncytiotrophoblast forms a continuous barrier between the maternal and fetal circulations. Here we present a serial block-face scanning electron microscopy (SBFSEM) study, based on a single image stack, showing pooling of fetal blood underneath a region of stretched syncytiotrophoblast that has become detached from the basement membrane. Erythrocytes are protruding from discrete holes in the syncytiotrophoblast suggesting that, under specific circumstances, the syncytiotrophoblast may be permeable to fetal cells. This observation represents a pathological process but it poses questions about the physical properties and permeability of the syncytiotrophoblast and may represent an early stage in the formation of fibrin deposits in areas of syncytial denudation. This study also illustrates how the 3D images generated by SBFSEM allow the interpretation of structures that could not be understood from a single histological section.

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Accepted/In Press date: 22 May 2017
e-pub ahead of print date: 17 July 2017

Identifiers

Local EPrints ID: 412074
URI: http://eprints.soton.ac.uk/id/eprint/412074
ISSN: 0021-8782
PURE UUID: 0628c527-db86-45e7-833f-e64c4bbf2cec
ORCID for Emma Lofthouse: ORCID iD orcid.org/0000-0002-0175-5590
ORCID for Bram Sengers: ORCID iD orcid.org/0000-0001-5859-6984
ORCID for Jane Cleal: ORCID iD orcid.org/0000-0001-7978-4327
ORCID for Rohan Lewis: ORCID iD orcid.org/0000-0003-4044-9104

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Date deposited: 06 Jul 2017 16:30
Last modified: 18 Feb 2021 17:20

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Contributors

Author: Eleni Palaiologou
Author: Patricia, Mary Goggin
Author: David Chatelet
Author: Emma Lofthouse ORCID iD
Author: Christopher Torrens
Author: Bram Sengers ORCID iD
Author: Jane Cleal ORCID iD
Author: Anton Page
Author: Rohan Lewis ORCID iD

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