Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography
Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography
A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth colocalization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.
Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.
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Chen, Mingzhou
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Mas, Josep
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Lindsey, Forbes
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Andrews, Melissa
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Dholakia, Kishan
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January 2018
Chen, Mingzhou
ca9498d2-9387-445e-89be-1d8a58fbe276
Mas, Josep
b3b007e9-9b71-4d7d-bfd3-71b7e4b7fda4
Lindsey, Forbes
c6278c56-8425-4cd7-a28a-1fb41b4114f3
Andrews, Melissa
ae987a2f-878e-4ae3-a7a3-a7170712096c
Dholakia, Kishan
c649b0a9-ab68-4571-b8c7-e84553d14174
Chen, Mingzhou, Mas, Josep, Lindsey, Forbes, Andrews, Melissa and Dholakia, Kishan
(2018)
Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography.
Journal of Biophotonics, 11 (1), , [e201700129].
(doi:10.1002/jbio.201700129).
Abstract
A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth colocalization obtained from the OCT, we can penetrate 1.2mm deep into the strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (Polystyrene) with a spatial offset.
Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.
Text
wmsors
- Accepted Manuscript
More information
Accepted/In Press date: 7 July 2017
e-pub ahead of print date: 17 August 2017
Published date: January 2018
Identifiers
Local EPrints ID: 412133
URI: http://eprints.soton.ac.uk/id/eprint/412133
ISSN: 1864-063X
PURE UUID: d57b687a-cad9-44c7-a843-a2335fa4381e
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Date deposited: 11 Jul 2017 16:31
Last modified: 16 Mar 2024 05:31
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Contributors
Author:
Mingzhou Chen
Author:
Josep Mas
Author:
Forbes Lindsey
Author:
Kishan Dholakia
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