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Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared

Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared
Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared
Mid-infrared (MIR) spectroscopy is a powerful tool for characterising the vibrations of molecular bonds and is therefore ideal for label-free detection of chemical species. Recent research into thin-film deposition and etching techniques for mid-infrared materials shows potential for realising miniaturised bedside biosensors for clinical diagnostics exploiting MIR spectroscopy, to replace laboratory based-techniques. However, lack of refractive index information for commonly encountered biological media and analytes hampers optimisation of biosensor performance for maximum sensitivity, especially for devices exploiting evanescent spectroscopy. Here we present refractive index data for human whole blood and several aqueous solutions of general interest to the clinical community: anticoagulants, analgesics and buffers. The refractive indices are generally dominated by the water content of each sample and the whole blood spectra exhibit additional strong features due to protein content. Furthermore, we present a generalised method for extracting complex refractive indices of aqueous solutions in the mid-infrared region using conventional attenuated total reflection Fourier transform spectroscopy (ATR-FTIR) without the need for collimated or polarised incident light, as is required for existing methods.
2045-2322
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca
Smith, David
217b19f1-7588-4d54-a062-8394eea6dea1
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca
Smith, David
217b19f1-7588-4d54-a062-8394eea6dea1

Rowe, David, Wilkinson, James and Smith, David (2017) Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared. Scientific Reports, 7, [7356]. (doi:10.1038/s41598-017-07842-0).

Record type: Article

Abstract

Mid-infrared (MIR) spectroscopy is a powerful tool for characterising the vibrations of molecular bonds and is therefore ideal for label-free detection of chemical species. Recent research into thin-film deposition and etching techniques for mid-infrared materials shows potential for realising miniaturised bedside biosensors for clinical diagnostics exploiting MIR spectroscopy, to replace laboratory based-techniques. However, lack of refractive index information for commonly encountered biological media and analytes hampers optimisation of biosensor performance for maximum sensitivity, especially for devices exploiting evanescent spectroscopy. Here we present refractive index data for human whole blood and several aqueous solutions of general interest to the clinical community: anticoagulants, analgesics and buffers. The refractive indices are generally dominated by the water content of each sample and the whole blood spectra exhibit additional strong features due to protein content. Furthermore, we present a generalised method for extracting complex refractive indices of aqueous solutions in the mid-infrared region using conventional attenuated total reflection Fourier transform spectroscopy (ATR-FTIR) without the need for collimated or polarised incident light, as is required for existing methods.

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Complex refractive index spectra of whole blood and aqueous solutions of anticoagulants, analgesics and buffers in the mid-infrared - Accepted Manuscript
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s41598-017-07842-0 - Version of Record
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More information

Accepted/In Press date: 4 July 2017
e-pub ahead of print date: 4 August 2017

Identifiers

Local EPrints ID: 412134
URI: http://eprints.soton.ac.uk/id/eprint/412134
ISSN: 2045-2322
PURE UUID: 33fe9e39-f8ae-4309-ab5c-42c8151d83ce
ORCID for David Rowe: ORCID iD orcid.org/0000-0002-1167-150X
ORCID for James Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 11 Jul 2017 16:31
Last modified: 18 Feb 2021 17:20

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