The University of Southampton
University of Southampton Institutional Repository

High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation

High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation
High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation
In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packed with sandy loam soil at 1.2 g cm-3 36 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions. - We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 μm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. - Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.
image-based modelling,, non-invasive imaging,, rhizosphere, root hairs,, soil structure,, synchrotron, Hordeum vulgareL.
0028-646X
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Daly, Keith
29920932-1779-4d08-81f8-bdd898191e5a
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
George, TImothy S.
6b957175-f0b0-4456-87e3-241040c31cf8
Brown, Lawrie
48fbf377-f988-4ede-a97e-84e1c2870a45
Raffan, Annette
65909bd1-df87-4fc1-8c15-c2395d01dd0f
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Naveed, Muhammad
ffa04925-7d0a-42fa-b95b-548fe3e4e2db
Bengough, Anthony G.
ac1f754e-002c-44f6-8907-8771a4cd1fd3
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Hallett, Paul D.
2e6a4596-83d9-4aeb-bb84-025c3660a536
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Koebernick, Nicolai
118c4e45-02d8-42da-84c8-8ee4fac140ad
Daly, Keith
29920932-1779-4d08-81f8-bdd898191e5a
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
George, TImothy S.
6b957175-f0b0-4456-87e3-241040c31cf8
Brown, Lawrie
48fbf377-f988-4ede-a97e-84e1c2870a45
Raffan, Annette
65909bd1-df87-4fc1-8c15-c2395d01dd0f
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Naveed, Muhammad
ffa04925-7d0a-42fa-b95b-548fe3e4e2db
Bengough, Anthony G.
ac1f754e-002c-44f6-8907-8771a4cd1fd3
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Hallett, Paul D.
2e6a4596-83d9-4aeb-bb84-025c3660a536
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Koebernick, Nicolai, Daly, Keith, Keyes, Samuel, George, TImothy S., Brown, Lawrie, Raffan, Annette, Cooper, Laura, Naveed, Muhammad, Bengough, Anthony G., Sinclair, Ian, Hallett, Paul D. and Roose, Tiina (2017) High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation. New Phytologist. (doi:10.1111/nph.14705).

Record type: Article

Abstract

In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packed with sandy loam soil at 1.2 g cm-3 36 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions. - We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 μm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. - Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.

Text
s1-ln2680534218582232741703867393Hwf-1771574461IdV-16951475526805342FIRST_LOOK_PDF0001 - Accepted Manuscript
Download (2MB)

More information

Accepted/In Press date: 13 June 2017
e-pub ahead of print date: 31 July 2017
Keywords: image-based modelling,, non-invasive imaging,, rhizosphere, root hairs,, soil structure,, synchrotron, Hordeum vulgareL.

Identifiers

Local EPrints ID: 412143
URI: http://eprints.soton.ac.uk/id/eprint/412143
ISSN: 0028-646X
PURE UUID: 0852317e-5728-4283-a5ed-8ed9fb59f4f7
ORCID for Samuel Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Laura Cooper: ORCID iD orcid.org/0000-0003-0198-7591
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 11 Jul 2017 16:31
Last modified: 07 Oct 2020 01:59

Export record

Altmetrics

Contributors

Author: Nicolai Koebernick
Author: Keith Daly
Author: Samuel Keyes ORCID iD
Author: TImothy S. George
Author: Lawrie Brown
Author: Annette Raffan
Author: Laura Cooper ORCID iD
Author: Muhammad Naveed
Author: Anthony G. Bengough
Author: Ian Sinclair
Author: Paul D. Hallett
Author: Tiina Roose ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×