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There is no evidence to support literature claims of direct electron transfer (DET) for native glucose oxidase (GOx) at carbon nanotubes or graphene

There is no evidence to support literature claims of direct electron transfer (DET) for native glucose oxidase (GOx) at carbon nanotubes or graphene
There is no evidence to support literature claims of direct electron transfer (DET) for native glucose oxidase (GOx) at carbon nanotubes or graphene
It is widely claimed that native GOx undergoes direct electron transfer (DET) at nanostructured electrodes. In this paper we argue that the vast majority, if not all, of these claims are incorrect. We present results for GOx adsorbed on MWCNTs, a typical nanostructured electrode. We show that the surface redox peaks usually attributed to DET to GOx actually arise from flavin, and possibly catalase, impurities present in the as supplied commercial enzyme that are adsorbed at the electrode surface. We show that the observed response to glucose is due to enzymatic activity, but not electroactivity, of adsorbed GOx that catalyses the reaction of D-glucose with dissolved oxygen leading to a decrease in the oxygen reduction current that correlates with the glucose concentration.
Enzyme electrochemistry, glucose oxidase, direct electron transfer
1572-6657
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Al-Lolage, Firas A.
83184275-ce0e-4887-aee4-4b4c81bf7c06
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Al-Lolage, Firas A.
83184275-ce0e-4887-aee4-4b4c81bf7c06

Bartlett, Philip N. and Al-Lolage, Firas A. (2017) There is no evidence to support literature claims of direct electron transfer (DET) for native glucose oxidase (GOx) at carbon nanotubes or graphene. Journal of Electroanalytical Chemistry. (doi:10.1016/j.jelechem.2017.06.021).

Record type: Article

Abstract

It is widely claimed that native GOx undergoes direct electron transfer (DET) at nanostructured electrodes. In this paper we argue that the vast majority, if not all, of these claims are incorrect. We present results for GOx adsorbed on MWCNTs, a typical nanostructured electrode. We show that the surface redox peaks usually attributed to DET to GOx actually arise from flavin, and possibly catalase, impurities present in the as supplied commercial enzyme that are adsorbed at the electrode surface. We show that the observed response to glucose is due to enzymatic activity, but not electroactivity, of adsorbed GOx that catalyses the reaction of D-glucose with dissolved oxygen leading to a decrease in the oxygen reduction current that correlates with the glucose concentration.

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GOx_DET_18_5_17_docx - Accepted Manuscript
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GOx DET Table Final
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More information

Accepted/In Press date: 12 June 2017
e-pub ahead of print date: 13 June 2017
Keywords: Enzyme electrochemistry, glucose oxidase, direct electron transfer

Identifiers

Local EPrints ID: 412283
URI: https://eprints.soton.ac.uk/id/eprint/412283
ISSN: 1572-6657
PURE UUID: 5679b6a8-6867-486d-b9ea-996cb301c7c3
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 17 Jul 2017 13:26
Last modified: 14 Mar 2019 05:49

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