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Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells

Record type: Article

"Light-up" probes, icosa-alpha-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-alpha-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.

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Citation

Privat, Eric, Melvin, Tracy, Asseline, Ulysse and Vigny, Paul (2001) Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells Photochemistry and Photobiology, 74, (4), pp. 532-541.

More information

Published date: October 2001

Identifiers

Local EPrints ID: 41236
URI: http://eprints.soton.ac.uk/id/eprint/41236
ISSN: 0031-8655
PURE UUID: a40cf06b-401e-4557-8844-646a1c5d675b

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Date deposited: 04 Aug 2006
Last modified: 17 Jul 2017 15:32

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Contributors

Author: Eric Privat
Author: Tracy Melvin
Author: Ulysse Asseline
Author: Paul Vigny

University divisions


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