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Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells

Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells
Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells
"Light-up" probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-alpha-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.
0031-8655
532-541
Privat, Eric
cd59645e-cb14-494f-bfa0-eb5fa5474d73
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Asseline, Ulysse
543d0dd8-2d4f-44fb-a9e3-7a4d6795d704
Vigny, Paul
d06c1221-9686-4f05-872b-454d5f6cdee5
Privat, Eric
cd59645e-cb14-494f-bfa0-eb5fa5474d73
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Asseline, Ulysse
543d0dd8-2d4f-44fb-a9e3-7a4d6795d704
Vigny, Paul
d06c1221-9686-4f05-872b-454d5f6cdee5

Privat, Eric, Melvin, Tracy, Asseline, Ulysse and Vigny, Paul (2001) Oligonucleotide-conjugated thiazole orange probes as "light-up" probes for messenger ribonucleic acid molecules in living cells. Photochemistry and Photobiology, 74 (4), 532-541.

Record type: Article

Abstract

"Light-up" probes, icosa-α-thymidylate-thiazole orange conjugates, for the in situ time-resolved detection of messenger ribonucleic acid (mRNA) in living cells are evaluated. Upon annealing with polyA in aqueous solutions, the icosa-alpha-thymidylate-thiazole orange conjugates were shown to be up to 15 times more fluorescent. Microinjection of these probes into adherent fibroblasts resulted in high yields of hybridization and fluorescent signals. Incubation of cells in the presence of these probes resulted in facile internalization of the probe and similar painting of the messenger RNA in the nuclear and cytosolic regions.

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Published date: October 2001

Identifiers

Local EPrints ID: 41236
URI: http://eprints.soton.ac.uk/id/eprint/41236
ISSN: 0031-8655
PURE UUID: a40cf06b-401e-4557-8844-646a1c5d675b

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Date deposited: 04 Aug 2006
Last modified: 07 Apr 2020 16:37

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Contributors

Author: Eric Privat
Author: Tracy Melvin
Author: Ulysse Asseline
Author: Paul Vigny

University divisions

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