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Biological behaviour of thin films consisting of Au nanoparticles dispersed in a TiO2 dielectric matrix

Biological behaviour of thin films consisting of Au nanoparticles dispersed in a TiO2 dielectric matrix
Biological behaviour of thin films consisting of Au nanoparticles dispersed in a TiO2 dielectric matrix
In this work it was studied the possible use of thin films, composed of Au nanoparticles (NPs) embedded in a TiO2 matrix, in biological applications, by evaluating their interaction with a well-known protein, Bovine Serum Albumin (BSA), as well as with microbial cells (Candida albicans). The films were produced by one-step reactive DC magnetron sputtering followed by heat-treatment. The samples revealed a composition of 8.3 at.% of Au and a stoichiometric TiO2 matrix. The annealing promoted grain size increase of the Au NPs from 3 nm (at 300 °C) to 7 nm (at 500 °C) and a progressive crystallization of the TiO2 matrix to anatase. A broad localized surface plasmon resonance (LSPR) absorption band (λ = 580–720 nm) was clearly observed in the sample annealed at 500 °C, being less intense at 300 °C. The biological tests indicated that the BSA adhesion is dependent on surface nanostructure morphology, which in turn depends on the annealing temperature that changed the roughness and wettability of the films. The Au:TiO2 thin films also induced a significant change of the microbial cell membrane integrity, and ultimately the cell viability, which in turn affected the adhesion on its surface. The microstructural changes (structure, grain size and surface morphology) of the Au:TiO2 films promoted by heat-treatment shaped the amount of BSA adhered and affected cell viability.
0042-207X
360-368
Borges, J.
c8e5c89d-68cf-454e-a7c3-4f4629f32120
Costa, D.
a3be0f6b-2273-42fa-a6cd-07bbd945365f
Antunes, E
f134d48c-7efa-4917-937b-79a5bb77a9c3
Lopes, C.
75ae989a-13d8-46e4-9781-14d7dfc32045
Rodrigues, M.S.
8ee697c5-af21-45e7-b905-a1f76b4c0b47
Apreutesei, M
88e5f981-ab70-4b6f-8948-cb8362c137b2
Alves, E.
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Barradas, N.P.
8408c4b4-b978-4b33-be8a-99eaf6add1ca
Pedrosa, P.
4e26f035-75f1-45c0-a6ff-0a879b52b2fd
Moura, C
3dd7eb32-f5e7-44dd-9b40-6eba803261fb
Cunha, L
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Polcar, Tomas
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Vas, F
fc30b532-41d5-47f6-a215-1c48a7a1a20a
Sampaio, P
c9c36ab7-7381-48de-b8fe-3415c93b3870
Borges, J.
c8e5c89d-68cf-454e-a7c3-4f4629f32120
Costa, D.
a3be0f6b-2273-42fa-a6cd-07bbd945365f
Antunes, E
f134d48c-7efa-4917-937b-79a5bb77a9c3
Lopes, C.
75ae989a-13d8-46e4-9781-14d7dfc32045
Rodrigues, M.S.
8ee697c5-af21-45e7-b905-a1f76b4c0b47
Apreutesei, M
88e5f981-ab70-4b6f-8948-cb8362c137b2
Alves, E.
690fff18-b1ff-43a3-85fa-15f04a997f0c
Barradas, N.P.
8408c4b4-b978-4b33-be8a-99eaf6add1ca
Pedrosa, P.
4e26f035-75f1-45c0-a6ff-0a879b52b2fd
Moura, C
3dd7eb32-f5e7-44dd-9b40-6eba803261fb
Cunha, L
48f797a4-2da8-4763-8fa0-392b878aba2d
Polcar, Tomas
c669b663-3ba9-4e7b-9f97-8ef5655ac6d2
Vas, F
fc30b532-41d5-47f6-a215-1c48a7a1a20a
Sampaio, P
c9c36ab7-7381-48de-b8fe-3415c93b3870

Borges, J., Costa, D., Antunes, E, Lopes, C., Rodrigues, M.S., Apreutesei, M, Alves, E., Barradas, N.P., Pedrosa, P., Moura, C, Cunha, L, Polcar, Tomas, Vas, F and Sampaio, P (2015) Biological behaviour of thin films consisting of Au nanoparticles dispersed in a TiO2 dielectric matrix. Vacuum, 122 (B), 360-368. (doi:10.1016/j.vacuum.2015.03.036).

Record type: Article

Abstract

In this work it was studied the possible use of thin films, composed of Au nanoparticles (NPs) embedded in a TiO2 matrix, in biological applications, by evaluating their interaction with a well-known protein, Bovine Serum Albumin (BSA), as well as with microbial cells (Candida albicans). The films were produced by one-step reactive DC magnetron sputtering followed by heat-treatment. The samples revealed a composition of 8.3 at.% of Au and a stoichiometric TiO2 matrix. The annealing promoted grain size increase of the Au NPs from 3 nm (at 300 °C) to 7 nm (at 500 °C) and a progressive crystallization of the TiO2 matrix to anatase. A broad localized surface plasmon resonance (LSPR) absorption band (λ = 580–720 nm) was clearly observed in the sample annealed at 500 °C, being less intense at 300 °C. The biological tests indicated that the BSA adhesion is dependent on surface nanostructure morphology, which in turn depends on the annealing temperature that changed the roughness and wettability of the films. The Au:TiO2 thin films also induced a significant change of the microbial cell membrane integrity, and ultimately the cell viability, which in turn affected the adhesion on its surface. The microstructural changes (structure, grain size and surface morphology) of the Au:TiO2 films promoted by heat-treatment shaped the amount of BSA adhered and affected cell viability.

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Published date: 11 April 2015

Identifiers

Local EPrints ID: 412416
URI: https://eprints.soton.ac.uk/id/eprint/412416
ISSN: 0042-207X
PURE UUID: 49c2464b-a907-4051-aa08-58c38f6cd3b0
ORCID for Tomas Polcar: ORCID iD orcid.org/0000-0002-0863-6287

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Date deposited: 17 Jul 2017 13:41
Last modified: 20 Jul 2019 00:43

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