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Links between methane oxidation rates and methanotroph community composition in a pine forest soil

Links between methane oxidation rates and methanotroph community composition in a pine forest soil
Links between methane oxidation rates and methanotroph community composition in a pine forest soil
The main gap in our knowledge about what determines the rate of CH4 oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following 13CH4 incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH4 oxidation rates. The rates of 13C incorporation into PLFAs differed between horizons, with low 13C incorporation in the organic soil and relatively high 13C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the 13C label also differed between horizons, and statistical analyses suggested that the methanotroph community composition was a major cause of variation in CH4 oxidation rates. Both PLFA and pmoA-based data indicated that CH4 oxidizers in this soil belong to the uncultivated ‘upland soil cluster α’. CH4 oxidation potential exhibited the opposite pattern to 13C incorporation, suggesting that CH4 oxidation potential assays may correlate poorly with in situ oxidation rates. The DNA/RNA-SIP assay was not successful, most likely due to insufficient 13C-incorporation into DNA/RNA. The limitations of the technique are briefly discussed.
356-366
Bengtson, Per
ff2579af-4f3a-4203-a761-923a8263aee1
Basiliko, Nathan
da4eaa78-4c38-48b7-b2cb-3fc98ca24394
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Hills, Melissa
b3c4ec07-2bd2-46a9-95fa-feab07a52e62
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9
Roy, R.
01b0f297-1ca5-4754-a486-11c47c4f0e4c
Grayston, Sue
021597c0-dcda-4180-992d-d21ddf1c4238
Bengtson, Per
ff2579af-4f3a-4203-a761-923a8263aee1
Basiliko, Nathan
da4eaa78-4c38-48b7-b2cb-3fc98ca24394
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Hills, Melissa
b3c4ec07-2bd2-46a9-95fa-feab07a52e62
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9
Roy, R.
01b0f297-1ca5-4754-a486-11c47c4f0e4c
Grayston, Sue
021597c0-dcda-4180-992d-d21ddf1c4238

Bengtson, Per, Basiliko, Nathan, Dumont, Marc, Hills, Melissa, Murrell, J. Colin, Roy, R. and Grayston, Sue (2009) Links between methane oxidation rates and methanotroph community composition in a pine forest soil. FEMS microbiology ecology, 70, 356-366.

Record type: Article

Abstract

The main gap in our knowledge about what determines the rate of CH4 oxidation in forest soils is the biology of the microorganisms involved, the identity of which remains unclear. In this study, we used stable-isotope probing (SIP) following 13CH4 incorporation into phospholipid fatty acids (PLFAs) and DNA/RNA, and sequencing of methane mono-oxygenase (pmoA) genes, to identify the influence of variation in community composition on CH4 oxidation rates. The rates of 13C incorporation into PLFAs differed between horizons, with low 13C incorporation in the organic soil and relatively high 13C incorporation into the two mineral horizons. The microbial community composition of the methanotrophs incorporating the 13C label also differed between horizons, and statistical analyses suggested that the methanotroph community composition was a major cause of variation in CH4 oxidation rates. Both PLFA and pmoA-based data indicated that CH4 oxidizers in this soil belong to the uncultivated ‘upland soil cluster α’. CH4 oxidation potential exhibited the opposite pattern to 13C incorporation, suggesting that CH4 oxidation potential assays may correlate poorly with in situ oxidation rates. The DNA/RNA-SIP assay was not successful, most likely due to insufficient 13C-incorporation into DNA/RNA. The limitations of the technique are briefly discussed.

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Published date: October 2009

Identifiers

Local EPrints ID: 412447
URI: https://eprints.soton.ac.uk/id/eprint/412447
PURE UUID: 4533a8db-fcc8-480f-957e-9ac694f87af4
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 17 Jul 2017 13:47
Last modified: 14 Mar 2019 01:31

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