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Involvement of MmoR and MmoG in the transcriptional activation of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b

Involvement of MmoR and MmoG in the transcriptional activation of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b
Involvement of MmoR and MmoG in the transcriptional activation of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b
Methanotrophs oxidize methane to methanol using the enzyme methane monooxygenase. Methylosinus trichosporium OB3b has two such enzymes: a membrane-bound particulate methane monooxygenase (pMMO) and a soluble, cytoplasmic methane monooxygenase (sMMO). In methanotrophs possessing both enzymes, the expression of the genes encoding sMMO and pMMO is regulated by copper ions, with sMMO expressed solely when copper is limiting. Virtually nothing is known about the specific machinery involved in the copper-regulated transcription
of mmo genes except the identification of two proteins necessary for the
expression: a s54-dependent transcriptional activator, MmoR, and a putative
GroEL-like chaperone, MmoG. Genes encoding mmoR and mmoG are located
immediately upstream of those encoding sMMO in the genome of M. trichosporium
OB3b. Here, we use a green fluorescent protein promoter probe vector to
show that nearly the complete intergenic DNA sequence between mmoG and
mmoX is absolutely required for transcriptional activation. Furthermore, we used
gel-shift assays to demonstrate that both MmoR and MmoG were required for
protein binding to this region of DNA.
0378-1097
181-187
Scanlan, Julie
76e5fb31-0ca6-4980-8b6e-20d5ee1ca27d
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9
Scanlan, Julie
76e5fb31-0ca6-4980-8b6e-20d5ee1ca27d
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Murrell, J. Colin
22f4dcaa-2fab-4b88-9365-0418bae53ec9

Scanlan, Julie, Dumont, Marc and Murrell, J. Colin (2009) Involvement of MmoR and MmoG in the transcriptional activation of soluble methane monooxygenase genes in Methylosinus trichosporium OB3b. FEMS Microbiology Letters, 301, 181-187. (doi:10.1111/j.1574-6968.2009.01816.x).

Record type: Article

Abstract

Methanotrophs oxidize methane to methanol using the enzyme methane monooxygenase. Methylosinus trichosporium OB3b has two such enzymes: a membrane-bound particulate methane monooxygenase (pMMO) and a soluble, cytoplasmic methane monooxygenase (sMMO). In methanotrophs possessing both enzymes, the expression of the genes encoding sMMO and pMMO is regulated by copper ions, with sMMO expressed solely when copper is limiting. Virtually nothing is known about the specific machinery involved in the copper-regulated transcription
of mmo genes except the identification of two proteins necessary for the
expression: a s54-dependent transcriptional activator, MmoR, and a putative
GroEL-like chaperone, MmoG. Genes encoding mmoR and mmoG are located
immediately upstream of those encoding sMMO in the genome of M. trichosporium
OB3b. Here, we use a green fluorescent protein promoter probe vector to
show that nearly the complete intergenic DNA sequence between mmoG and
mmoX is absolutely required for transcriptional activation. Furthermore, we used
gel-shift assays to demonstrate that both MmoR and MmoG were required for
protein binding to this region of DNA.

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Published date: October 2009

Identifiers

Local EPrints ID: 412448
URI: http://eprints.soton.ac.uk/id/eprint/412448
ISSN: 0378-1097
PURE UUID: 6720650b-ce23-4f6f-b534-140656b2c2a5
ORCID for Marc Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 17 Jul 2017 13:47
Last modified: 08 Jan 2022 03:25

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Contributors

Author: Julie Scanlan
Author: Marc Dumont ORCID iD
Author: J. Colin Murrell

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