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Development of a clay based bioink for 3D cell printing for skeletal application

Development of a clay based bioink for 3D cell printing for skeletal application
Development of a clay based bioink for 3D cell printing for skeletal application
Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs. However, it is still challenging to print structures with both, high shape fidelity and cell vitality. Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting. By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity. Following extrusion, approximately 70%–75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs. Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions. Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods. Laponite is known for its favourable drug delivery properties. Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink. We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite. In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.
1758-5082
Ahlfeld, T.
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Cidonio, Gianluca
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Kilian, D.
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Duin, S.
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Akkineni, A.R.
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Dawson, Jonathan
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Yang, Shoufeng
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Lode, A.
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Oreffo, Richard
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Gelinsky, M.
72546c96-7c96-430a-9862-75953a2678ea
Ahlfeld, T.
e7376b44-1dff-482e-ae3f-cabda3b294d7
Cidonio, Gianluca
558ad583-899a-4d8c-b42b-bc1c354c8757
Kilian, D.
03d4bb58-c31f-4d38-8663-2abd7e014e40
Duin, S.
42011d05-cee1-46b1-a297-218071fa4e6d
Akkineni, A.R.
c6f9fd84-2e34-4a5f-962b-0e5e46069afa
Dawson, Jonathan
b220fe76-498d-47be-9995-92da6c289cf3
Yang, Shoufeng
e0018adf-8123-4a54-b8dd-306c10ca48f1
Lode, A.
24eb4757-ec3e-4936-b3c4-0dc1d88faf88
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Gelinsky, M.
72546c96-7c96-430a-9862-75953a2678ea

Ahlfeld, T., Cidonio, Gianluca, Kilian, D., Duin, S., Akkineni, A.R., Dawson, Jonathan, Yang, Shoufeng, Lode, A., Oreffo, Richard and Gelinsky, M. (2017) Development of a clay based bioink for 3D cell printing for skeletal application. Biofabrication, 9 (3), [034103]. (doi:10.1088/1758-5090/aa7e96).

Record type: Article

Abstract

Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs. However, it is still challenging to print structures with both, high shape fidelity and cell vitality. Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting. By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity. Following extrusion, approximately 70%–75% of printed immortalised human mesenchymal stem cells survived and cell viability was maintained over 21 days within the plotted constructs. Mechanical properties of scaffolds comprised of the composite bioink decreased over time when stored under cell culture conditions. Nevertheless, shape of the plotted constructs was preserved even over longer cultivation periods. Laponite is known for its favourable drug delivery properties. Two model proteins, bovine serum albumin and vascular endothelial growth factor were loaded into the bioink. We demonstrate that the release of both growth factors significantly changed to a more sustained profile by inclusion of Laponite in comparison to an alginate-methylcellulose blend in the absence of Laponite. In summary, addition of a synthetic clay, Laponite, improved printability, increased shape fidelity and was beneficial for controlled release of biologically active agents such as growth factors.

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Accepted/In Press date: 10 July 2017
e-pub ahead of print date: 10 July 2017
Published date: September 2017

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Local EPrints ID: 412674
URI: http://eprints.soton.ac.uk/id/eprint/412674
ISSN: 1758-5082
PURE UUID: d1a6a1bf-cf56-4293-88ac-a1ae523aed73
ORCID for Jonathan Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Shoufeng Yang: ORCID iD orcid.org/0000-0002-3888-3211
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 25 Jul 2017 16:31
Last modified: 07 Oct 2020 06:07

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Contributors

Author: T. Ahlfeld
Author: D. Kilian
Author: S. Duin
Author: A.R. Akkineni
Author: Jonathan Dawson ORCID iD
Author: Shoufeng Yang ORCID iD
Author: A. Lode
Author: Richard Oreffo ORCID iD
Author: M. Gelinsky

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