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Consistency and variability of DNA methylation in women during puberty, young adulthood, and pregnancy

Consistency and variability of DNA methylation in women during puberty, young adulthood, and pregnancy
Consistency and variability of DNA methylation in women during puberty, young adulthood, and pregnancy
Prior DNA methylation (DNA-m) analyses have identified cytosine-phosphate-guanine (CpG) sites, which show either a significant change or consistency during lifetime. However, the proportion of CpGs that are neither significantly different nor consistent over time (indifferent CpGs) is unknown. We investigated the methylation dynamics, both longitudinal changes and consistency, in women from preadolescence to late pregnancy using DNA-m of peripheral blood cells. Consistency of cell type–adjusted DNA-m between paired individuals was assessed by regressing CpGs of subsequent age on the prior, stability by intraclass correlation coefficients (>0.5), and changes by linear mixed models. In the first 2 transitions (10-18 years and 18 years to early pregnancy), 19.5% and 20.9% CpGs were consistent, but only 0.35% in the third transition (from early to late pregnancy). Significant changes in methylation were found in 0.7%, 5.6%, and 0% CpGs, respectively. Functional enrichment analyses of genes with significant changes in DNA-m in early pregnancy (5.6%) showed that the maternal DNA-m seems to reflect signaling pathways between the uterus and the trophoblast. The transition from early to late pregnancy showed low consistency/stability and no changes, suggesting the presence of a large proportion of indifferent CpGs in late pregnancy.
Chen, Su
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Mukherjee, Nandini
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Janjanam, Vimala
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Arshad, S. Hasan
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Kurukulaaratchy, Ramesh J.
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Holloway, John W.
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Zhang, Hongmei
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Karmaus, Wilfried
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Chen, Su
1d593b05-805d-46d4-b54e-96d2b4a4702e
Mukherjee, Nandini
f64f02d6-2fd0-40db-88ee-5f85b59b8e0b
Janjanam, Vimala
62f16860-3d75-4efc-b6b6-2a3a88549a85
Arshad, S. Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Kurukulaaratchy, Ramesh J.
9c7b8105-2892-49f2-8775-54d4961e3e74
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853

Chen, Su, Mukherjee, Nandini, Janjanam, Vimala, Arshad, S. Hasan, Kurukulaaratchy, Ramesh J., Holloway, John W., Zhang, Hongmei and Karmaus, Wilfried (2017) Consistency and variability of DNA methylation in women during puberty, young adulthood, and pregnancy. Genetics & Epigenetics, 9 (0). (doi:10.1177/1179237X17721540).

Record type: Article

Abstract

Prior DNA methylation (DNA-m) analyses have identified cytosine-phosphate-guanine (CpG) sites, which show either a significant change or consistency during lifetime. However, the proportion of CpGs that are neither significantly different nor consistent over time (indifferent CpGs) is unknown. We investigated the methylation dynamics, both longitudinal changes and consistency, in women from preadolescence to late pregnancy using DNA-m of peripheral blood cells. Consistency of cell type–adjusted DNA-m between paired individuals was assessed by regressing CpGs of subsequent age on the prior, stability by intraclass correlation coefficients (>0.5), and changes by linear mixed models. In the first 2 transitions (10-18 years and 18 years to early pregnancy), 19.5% and 20.9% CpGs were consistent, but only 0.35% in the third transition (from early to late pregnancy). Significant changes in methylation were found in 0.7%, 5.6%, and 0% CpGs, respectively. Functional enrichment analyses of genes with significant changes in DNA-m in early pregnancy (5.6%) showed that the maternal DNA-m seems to reflect signaling pathways between the uterus and the trophoblast. The transition from early to late pregnancy showed low consistency/stability and no changes, suggesting the presence of a large proportion of indifferent CpGs in late pregnancy.

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Chen S et al. geentics and Epigenetics 2017 Consistency and variabiltiy - Version of Record
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Accepted/In Press date: 3 May 2017
e-pub ahead of print date: 28 July 2017

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Local EPrints ID: 412809
URI: https://eprints.soton.ac.uk/id/eprint/412809
PURE UUID: a1dbc576-f8bb-4730-a207-6b3e0660a061
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

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Date deposited: 02 Aug 2017 16:30
Last modified: 03 Dec 2019 01:58

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