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Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting

Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting
Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting
The capacity of bone and cartilage to regenerate can be attributed to skeletal stem cells (SSCs) that reside within the bone marrow (BM). Given SSCs are rare and lack specific surface markers, antibody-based sorting has failed to deliver the cell purity required for clinical translation. Microfluidics offers new methods of isolating cells based on biophysical features including, but not limited to, size, electrical properties and stiffness. Here we report the characterisation of the dielectric properties of unexpanded SSCs using single-cell microfluidic impedance cytometry (MIC). Unexpanded SSCs had a mean size of 9.0 μm; larger than the majority of BM cells. During expansion, often used to purify and increase the number of SSCs, cell size and membrane capacitance increased significantly, highlighting the importance of characterising unaltered SSCs. In addition, MIC was used to track the osteogenic differentiation of SSCs and showed an increased membrane capacitance with differentiation. The electrical properties of primary SSCs were indistinct from other BM cells precluding its use as an isolation method. However, the current studies indicate that cell size in combination with another biophysical parameter, such as stiffness, could be used to design label-free devices for sorting SSCs with significant clinical impact.
Skeletal Stem Cells, Cell Sorting, Impedance Cytometry , Tissue Regeneration, Microfluidics, Label-free
1742-5689
1-13
Xavier, Miguel
23a18e02-6cee-42ff-8ee5-33a99fe5c565
de Andrés, María C.
9b3834e7-972f-410d-a8cb-199abd035b87
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Xavier, Miguel
23a18e02-6cee-42ff-8ee5-33a99fe5c565
de Andrés, María C.
9b3834e7-972f-410d-a8cb-199abd035b87
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Xavier, Miguel, de Andrés, María C., Spencer, Daniel, Oreffo, Richard and Morgan, Hywel (2017) Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting. Journal of the Royal Society Interface, 14 (133), 1-13. (doi:10.1098/rsif.2017.0233).

Record type: Article

Abstract

The capacity of bone and cartilage to regenerate can be attributed to skeletal stem cells (SSCs) that reside within the bone marrow (BM). Given SSCs are rare and lack specific surface markers, antibody-based sorting has failed to deliver the cell purity required for clinical translation. Microfluidics offers new methods of isolating cells based on biophysical features including, but not limited to, size, electrical properties and stiffness. Here we report the characterisation of the dielectric properties of unexpanded SSCs using single-cell microfluidic impedance cytometry (MIC). Unexpanded SSCs had a mean size of 9.0 μm; larger than the majority of BM cells. During expansion, often used to purify and increase the number of SSCs, cell size and membrane capacitance increased significantly, highlighting the importance of characterising unaltered SSCs. In addition, MIC was used to track the osteogenic differentiation of SSCs and showed an increased membrane capacitance with differentiation. The electrical properties of primary SSCs were indistinct from other BM cells precluding its use as an isolation method. However, the current studies indicate that cell size in combination with another biophysical parameter, such as stiffness, could be used to design label-free devices for sorting SSCs with significant clinical impact.

Text Size and Dielectric Properties of Skeletal Stem Cells - Accepted Manuscript
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More information

Accepted/In Press date: 27 July 2017
e-pub ahead of print date: 23 August 2017
Published date: August 2017
Keywords: Skeletal Stem Cells, Cell Sorting, Impedance Cytometry , Tissue Regeneration, Microfluidics, Label-free

Identifiers

Local EPrints ID: 413035
URI: https://eprints.soton.ac.uk/id/eprint/413035
ISSN: 1742-5689
PURE UUID: 95b67ed7-eff5-4cb4-9a29-e6b60fdb3806
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 14 Aug 2017 16:30
Last modified: 06 Jun 2018 12:53

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Contributors

Author: Miguel Xavier
Author: María C. de Andrés
Author: Daniel Spencer
Author: Richard Oreffo ORCID iD
Author: Hywel Morgan ORCID iD

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