F-group bZIPs in barley - a role in Zn deficiency
F-group bZIPs in barley - a role in Zn deficiency
Zinc (Zn) deficiency negatively impacts the development and health of plants and affects crop yield. When experiencing low Zn, plants undergo an adaptive response to maintain Zn homeostasis. We provide further evidence for the role of F-group transcription factors, AtbZIP19 and AtbZIP23, in responding to Zn deficiency in Arabidopsis and demonstrate the sensitivity and specificity of this response. Despite their economic importance, the role of F-group bZIPs in cereal crops is largely unknown. Here we provide new insights by functionally characterizing these in barley (Hordeum vulgare), demonstrating an expanded number of F-group bZIPs (seven) compared to Arabidopsis. The F-group barley bZIPs, HvbZIP56 and HvbZIP62, partially rescue the Zn-dependent growth phenotype and ZIP-transporter gene regulation of an Arabidopsis bzip19-4 bzip23-2 mutant. This supports a conserved mechanism of action in adapting to Zn deficiency. HvbZIP56 localizes to the cytoplasm and nucleus when expressed in Arabidopsis and tobacco. Promoter analysis demonstrates that the barley ZIP transporters that are up-regulated under Zn deficiency contain cis Zn-deficiency response elements (ZDREs). ZDREs are also found in particular barley bZIP promoters. This study represents a significant step forward in understanding the mechanisms controlling Zn responses in cereal crops, and will aid in developing strategies for crop improvement.
2754-2770
Nazri, Ahmad Zulhilmi
3f619b2a-7f30-44a0-b0a6-0c867b9afc37
Griffin, Jonathan H.C.
4d5fb0fd-5fd1-4bef-8412-0ae459c888e8
Peaston, Kerry A.
55c75e49-e55d-441c-a07e-867e1fa000e7
Alexander-Webber, Douglas G.A.
778e8d87-321e-453b-80a5-ebf0ecd87155
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
November 2017
Nazri, Ahmad Zulhilmi
3f619b2a-7f30-44a0-b0a6-0c867b9afc37
Griffin, Jonathan H.C.
4d5fb0fd-5fd1-4bef-8412-0ae459c888e8
Peaston, Kerry A.
55c75e49-e55d-441c-a07e-867e1fa000e7
Alexander-Webber, Douglas G.A.
778e8d87-321e-453b-80a5-ebf0ecd87155
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Nazri, Ahmad Zulhilmi, Griffin, Jonathan H.C., Peaston, Kerry A., Alexander-Webber, Douglas G.A. and Williams, Lorraine E.
(2017)
F-group bZIPs in barley - a role in Zn deficiency.
Plant, Cell & Environment, 40 (11), .
(doi:10.1111/pce.13045).
Abstract
Zinc (Zn) deficiency negatively impacts the development and health of plants and affects crop yield. When experiencing low Zn, plants undergo an adaptive response to maintain Zn homeostasis. We provide further evidence for the role of F-group transcription factors, AtbZIP19 and AtbZIP23, in responding to Zn deficiency in Arabidopsis and demonstrate the sensitivity and specificity of this response. Despite their economic importance, the role of F-group bZIPs in cereal crops is largely unknown. Here we provide new insights by functionally characterizing these in barley (Hordeum vulgare), demonstrating an expanded number of F-group bZIPs (seven) compared to Arabidopsis. The F-group barley bZIPs, HvbZIP56 and HvbZIP62, partially rescue the Zn-dependent growth phenotype and ZIP-transporter gene regulation of an Arabidopsis bzip19-4 bzip23-2 mutant. This supports a conserved mechanism of action in adapting to Zn deficiency. HvbZIP56 localizes to the cytoplasm and nucleus when expressed in Arabidopsis and tobacco. Promoter analysis demonstrates that the barley ZIP transporters that are up-regulated under Zn deficiency contain cis Zn-deficiency response elements (ZDREs). ZDREs are also found in particular barley bZIP promoters. This study represents a significant step forward in understanding the mechanisms controlling Zn responses in cereal crops, and will aid in developing strategies for crop improvement.
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Accepted/In Press date: 9 July 2017
e-pub ahead of print date: 1 August 2017
Published date: November 2017
Identifiers
Local EPrints ID: 413047
URI: http://eprints.soton.ac.uk/id/eprint/413047
ISSN: 0140-7791
PURE UUID: ea3f5638-6fa0-4529-8119-4b60ba93b154
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Date deposited: 14 Aug 2017 16:30
Last modified: 16 Mar 2024 05:36
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Author:
Ahmad Zulhilmi Nazri
Author:
Jonathan H.C. Griffin
Author:
Kerry A. Peaston
Author:
Douglas G.A. Alexander-Webber
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