Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides
Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides
Two imported thylakoid membrane proteins, PSII-X and PSII-W, are synthesised with cleavable N-terminal signal peptides that closely resemble those of Sec-dependent lumenal proteins. In this report we have reconstituted the insertion of pre-PSII-X and pre-PSII-W into isolated thylakoids. We show that insertion dots not require either nucleoside triphosphates or stromal extracts, both of which are required for Sec- and signal recognition particle (SRP)-dependent targeting mechanisms. Insertion is furthermore unaffected by protease treatments that destroy the known protein translocation apparatus in the thylakoid manbrane. We conclude that these membrane proteins are inserted by an unusual Sec/SRP-independent mechanism that probably resembles that used by CF(o)II, and we discuss possible parallels with the biogenesis of phage M13 procoat.
Chloroplast, Membrane protein, Sec, Signal peptide, Thylakoid membrane
105-108
Kim, Soo Jung
39c39977-8bfe-4894-bd72-c955b5ebb523
Robinson, Colin
678e0157-d628-44e8-83de-3591b07c673f
Mant, Alexandra
63319e45-deeb-45ad-a30d-e05b42052a0d
6 March 1998
Kim, Soo Jung
39c39977-8bfe-4894-bd72-c955b5ebb523
Robinson, Colin
678e0157-d628-44e8-83de-3591b07c673f
Mant, Alexandra
63319e45-deeb-45ad-a30d-e05b42052a0d
Kim, Soo Jung, Robinson, Colin and Mant, Alexandra
(1998)
Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides.
FEBS Letters, 424 (1-2), .
(doi:10.1016/S0014-5793(98)00148-3).
Abstract
Two imported thylakoid membrane proteins, PSII-X and PSII-W, are synthesised with cleavable N-terminal signal peptides that closely resemble those of Sec-dependent lumenal proteins. In this report we have reconstituted the insertion of pre-PSII-X and pre-PSII-W into isolated thylakoids. We show that insertion dots not require either nucleoside triphosphates or stromal extracts, both of which are required for Sec- and signal recognition particle (SRP)-dependent targeting mechanisms. Insertion is furthermore unaffected by protease treatments that destroy the known protein translocation apparatus in the thylakoid manbrane. We conclude that these membrane proteins are inserted by an unusual Sec/SRP-independent mechanism that probably resembles that used by CF(o)II, and we discuss possible parallels with the biogenesis of phage M13 procoat.
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Published date: 6 March 1998
Keywords:
Chloroplast, Membrane protein, Sec, Signal peptide, Thylakoid membrane
Identifiers
Local EPrints ID: 413065
URI: http://eprints.soton.ac.uk/id/eprint/413065
ISSN: 0014-5793
PURE UUID: 9ebd7091-522e-4e15-be73-fb60a6f8e83f
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Date deposited: 14 Aug 2017 16:31
Last modified: 16 Mar 2024 03:40
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Contributors
Author:
Soo Jung Kim
Author:
Colin Robinson
Author:
Alexandra Mant
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