Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that L-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for L-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K(+) antiport system and similar energy systems for the uptake of l-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657(T) (= LMG 22481(T) = CIP 108307(T)) and Logan B-1599(T) (LMG 22483(T) = CIP 108308(T)), respectively.

Amino Acid Transport Systems, Antarctic Regions, Bacterial Proteins, Bacterial Typing Techniques, Base Composition, DNA, Bacterial, DNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, Genes, rRNA, Glutamic Acid, Gram-Positive Endospore-Forming Rods, Hot Temperature, Hydrogen-Ion Concentration, Ion Transport, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome, RNA, Bacterial, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil Microbiology, Journal Article, Research Support, Non-U.S. Gov't

10.1099/ijs.0.63397-0

1466-5026

1039-50

Allan, R.N.

390a7d0a-38e1-410a-8dfe-c8ef8408f5e1

Lebbe, L.

d2d72914-61f1-41f5-b604-2d1f643bd687

Heyrman, J.

9c0d74d7-47b3-430f-8c04-9e78f0ced7e3

De Vos, P.

10767dac-b5b0-4bc0-974a-8a95a26041d6

Buchanan, C.J.

e7722d44-00f0-44b9-ae4f-e23e61b9b41e

Logan, N.A.

8f141af4-f75d-4f57-9940-997555be6cbc

May 2005

Allan, R.N.

390a7d0a-38e1-410a-8dfe-c8ef8408f5e1

Lebbe, L.

d2d72914-61f1-41f5-b604-2d1f643bd687

Heyrman, J.

9c0d74d7-47b3-430f-8c04-9e78f0ced7e3

De Vos, P.

10767dac-b5b0-4bc0-974a-8a95a26041d6

Buchanan, C.J.

e7722d44-00f0-44b9-ae4f-e23e61b9b41e

Logan, N.A.

8f141af4-f75d-4f57-9940-997555be6cbc

Allan, R.N., Lebbe, L., Heyrman, J., De Vos, P., Buchanan, C.J. and Logan, N.A. (2005) Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. International Journal of Systematic and Evolutionary Microbiology, 55 (Pt 3), 1039-50. (doi:10.1099/ijs.0.63397-0).

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Published date: May 2005

Keywords: Amino Acid Transport Systems, Antarctic Regions, Bacterial Proteins, Bacterial Typing Techniques, Base Composition, DNA, Bacterial, DNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, Genes, rRNA, Glutamic Acid, Gram-Positive Endospore-Forming Rods, Hot Temperature, Hydrogen-Ion Concentration, Ion Transport, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome, RNA, Bacterial, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil Microbiology, Journal Article, Research Support, Non-U.S. Gov't