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The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum)

The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum)
The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum)
Understanding the molecular basis of zinc (Zn) uptake and transport in staple cereal crops is critical for improving both Zn content and tolerance to low Zn soils. This study demonstrates the importance of F-group bZIP transcription factors and ZIP transporters in responses to Zn deficiency in wheat (Triticum aestivum). Seven F group TabZIP genes and fourteen ZIPs with homeologs were identified in hexaploid wheat. Promoter analysis revealed the presence of Zn-deficiency-response elements (ZDREs) in a number of the ZIPs. Functional complementation of the zrt1/zrt2 yeast mutant by TaZIP3, 6, 7, 9, and 13 supported a Zn-transport ability. Group F TabZIPs contain the group-defining cysteine-histidine rich motifs, which are the predicted binding site of Zn2+ in the Zn-deficiency response. Conservation of these motifs varied between the TabZIPs suggesting individual TabZIPs may have specific roles in the wheat Zn-homeostatic network. Increased expression in response to low Zn levels was observed for several of the wheat ZIPs and bZIPs; this varied temporally and spatially suggesting specific functions in the response mechanism. The ability of the group F TabZIPs to bind to specific ZDREs in the promoters of TaZIPs, indicates a conserved mechanism in monocots and dicots in responding to Zn deficiency. In support of this, TabZIPF1-7DL and TabZIPF4-7AL afforded a strong level of rescue to the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency. These results provide a greater understanding of Zn-homeostatic mechanisms in wheat, demonstrating an expanded repertoire of F group bZIP transcription factors adding to the complexity of Zn homeostasis.
0960-7412
291-304
Evens, Nicholas P.
a7d0e962-829f-482e-a00a-2ca1dc474462
Buchner, Peter
9cd3c4ae-157c-45e3-8545-9d928e6ecad8
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Hawkesford, Malcolm J.
e8031a97-464d-449b-a3d9-ed4a21fd66df
Evens, Nicholas P.
a7d0e962-829f-482e-a00a-2ca1dc474462
Buchner, Peter
9cd3c4ae-157c-45e3-8545-9d928e6ecad8
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Hawkesford, Malcolm J.
e8031a97-464d-449b-a3d9-ed4a21fd66df

Evens, Nicholas P., Buchner, Peter, Williams, Lorraine E. and Hawkesford, Malcolm J. (2017) The role of ZIP transporters and group F bZIP transcription factors in the Zn-deficiency response of wheat (Triticum aestivum). The Plant Journal, 92 (2), 291-304. (doi:10.1111/tpj.13655).

Record type: Article

Abstract

Understanding the molecular basis of zinc (Zn) uptake and transport in staple cereal crops is critical for improving both Zn content and tolerance to low Zn soils. This study demonstrates the importance of F-group bZIP transcription factors and ZIP transporters in responses to Zn deficiency in wheat (Triticum aestivum). Seven F group TabZIP genes and fourteen ZIPs with homeologs were identified in hexaploid wheat. Promoter analysis revealed the presence of Zn-deficiency-response elements (ZDREs) in a number of the ZIPs. Functional complementation of the zrt1/zrt2 yeast mutant by TaZIP3, 6, 7, 9, and 13 supported a Zn-transport ability. Group F TabZIPs contain the group-defining cysteine-histidine rich motifs, which are the predicted binding site of Zn2+ in the Zn-deficiency response. Conservation of these motifs varied between the TabZIPs suggesting individual TabZIPs may have specific roles in the wheat Zn-homeostatic network. Increased expression in response to low Zn levels was observed for several of the wheat ZIPs and bZIPs; this varied temporally and spatially suggesting specific functions in the response mechanism. The ability of the group F TabZIPs to bind to specific ZDREs in the promoters of TaZIPs, indicates a conserved mechanism in monocots and dicots in responding to Zn deficiency. In support of this, TabZIPF1-7DL and TabZIPF4-7AL afforded a strong level of rescue to the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency. These results provide a greater understanding of Zn-homeostatic mechanisms in wheat, demonstrating an expanded repertoire of F group bZIP transcription factors adding to the complexity of Zn homeostasis.

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Accepted/In Press date: 28 July 2017
e-pub ahead of print date: 17 September 2017
Published date: October 2017

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Local EPrints ID: 413120
URI: http://eprints.soton.ac.uk/id/eprint/413120
ISSN: 0960-7412
PURE UUID: 37506e4f-d185-40dc-b7db-a88cb3799c8b

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Date deposited: 15 Aug 2017 16:30
Last modified: 16 Mar 2024 05:37

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Contributors

Author: Nicholas P. Evens
Author: Peter Buchner
Author: Malcolm J. Hawkesford

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