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Gold nanoparticle interactions in human blood: a model evaluation

Gold nanoparticle interactions in human blood: a model evaluation
Gold nanoparticle interactions in human blood: a model evaluation
In this study, we investigated gold nanoparticle (AuNP) interactions in blood using thromboelastography as a rapid screening tool to monitor their influence on blood coagulation. 1.2 nM colloidal AuNPs ranging from 12 to 85 nm have no effect in the blood, however, 5 nM AuNPs demonstrate pro-thrombogenic concentration dependent effects with a reduction in clot formation. Size effects exhibit a non-linear trend with 45 and 85 nm particles resulting in a faster pro-thrombotic response. Clot strength decreased with AuNP size with the greatest reduction with 28 nm particles. We assessed AuNP interactions in the blood focusing on their biological activity. AuNP-RGD possessed pro-coagulant activities, while PEG-thiol, human fibrinogen and clopidogrel prevented blood clot formation and influenced platelet activity, and were more efficient when bound to nanocarriers than unbound ligands. Such tests could fill the knowledge gaps in thrombogenicity of NPs between in vitro test methods and predict in vivo haemocompatibility.
1549-9634
1531-1542
Ajdari, Niloofar
7a90c043-a630-4b60-92f6-b38b74c6ffcd
Vyas, Cian
d6dde9b5-3361-4052-9aa4-6ef750b7bd36
Bogan, Stephanie L.
d5656aae-32bc-469c-a947-7694f11d4c39
Lwaleed, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21
Cousins, Brian G.
b064d863-3551-4ad6-9276-885a1fb541e5
Ajdari, Niloofar
7a90c043-a630-4b60-92f6-b38b74c6ffcd
Vyas, Cian
d6dde9b5-3361-4052-9aa4-6ef750b7bd36
Bogan, Stephanie L.
d5656aae-32bc-469c-a947-7694f11d4c39
Lwaleed, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21
Cousins, Brian G.
b064d863-3551-4ad6-9276-885a1fb541e5

Ajdari, Niloofar, Vyas, Cian, Bogan, Stephanie L., Lwaleed, Bashir and Cousins, Brian G. (2017) Gold nanoparticle interactions in human blood: a model evaluation Nanomedicine Nanotechnology Biology and Medicine, pp. 1531-1542.

Record type: Article

Abstract

In this study, we investigated gold nanoparticle (AuNP) interactions in blood using thromboelastography as a rapid screening tool to monitor their influence on blood coagulation. 1.2 nM colloidal AuNPs ranging from 12 to 85 nm have no effect in the blood, however, 5 nM AuNPs demonstrate pro-thrombogenic concentration dependent effects with a reduction in clot formation. Size effects exhibit a non-linear trend with 45 and 85 nm particles resulting in a faster pro-thrombotic response. Clot strength decreased with AuNP size with the greatest reduction with 28 nm particles. We assessed AuNP interactions in the blood focusing on their biological activity. AuNP-RGD possessed pro-coagulant activities, while PEG-thiol, human fibrinogen and clopidogrel prevented blood clot formation and influenced platelet activity, and were more efficient when bound to nanocarriers than unbound ligands. Such tests could fill the knowledge gaps in thrombogenicity of NPs between in vitro test methods and predict in vivo haemocompatibility.

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More information

Accepted/In Press date: 31 January 2017
e-pub ahead of print date: 24 February 2017
Published date: May 2017

Identifiers

Local EPrints ID: 413126
URI: http://eprints.soton.ac.uk/id/eprint/413126
ISSN: 1549-9634
PURE UUID: ed2ef114-7269-4d9d-98c8-0ad67236b994

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Date deposited: 15 Aug 2017 16:31
Last modified: 31 Oct 2017 21:29

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Contributors

Author: Niloofar Ajdari
Author: Cian Vyas
Author: Stephanie L. Bogan
Author: Bashir Lwaleed
Author: Brian G. Cousins

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