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High dose of a conjugated linoleic acid mixture increases insulin resistance in rats fed either a low fat or a high fat diet

High dose of a conjugated linoleic acid mixture increases insulin resistance in rats fed either a low fat or a high fat diet
High dose of a conjugated linoleic acid mixture increases insulin resistance in rats fed either a low fat or a high fat diet
Obesity and related diseases are becoming more prevalent. Conjugated linoleic acid (CLA) might be a useful coadjutant treatment helping to decrease fat mass. However, the precise impact of CLA is unclear because the decreased body fat mass is followed by an increase in insulin resistance. This study aimed to evaluate some of the consequences of a high dose of CLA in rats fed a normal low fat or a high fat diet for 30 days. Male Wistar rats were separated into 4 groups (each n = 10): Control group receiving 7% fat (soybean oil); CLA group receiving 4% soybean oil and 3% CLA mixture; animal fat (AF) group, receiving 45% fat (lard); and animal fat plus CLA (AF+CLA) group, receiving 42% lard and 3% CLA mixture. The CLA mixture contained 39.32 mole% c9,t11-CLA and 40.50 mole% t10,c12-CLA. After 30 days, both CLA groups (CLA and AF+CLA groups) developed insulin resistance, with an increase in glucose in the fasting state and in an insulin tolerance test. The CLA group had increased liver weight and percentage of saturated fatty acids in liver and adipose tissue. Feeding the high fat diet resulted in increased hepatic triacylglycerol accumulation and this was exacerbated by dietary CLA. It is concluded that a high dose of CLA mixture increases insulin resistance and exacerbates hepatic steatosis when combined with a high fat diet.
0947-7349
Bezan, Priscila
44d41e16-f19f-4667-ac1a-254f0b5d13cd
Holland, Héric
a6153bf6-98c6-4cdf-8f45-52a293eb4389
de Castro, Gabriela
157d4355-6a45-410b-b1db-bc9a74515a0a
Cardoso, João
709f6b15-e7e4-4352-9d74-b516f60379e1
Ovidio, Paula
766b939d-1d1c-4b1e-bb4a-2a08f98d2843
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jordao, Alceu
3b299005-a0bf-4fb6-8850-8a5258178203
Bezan, Priscila
44d41e16-f19f-4667-ac1a-254f0b5d13cd
Holland, Héric
a6153bf6-98c6-4cdf-8f45-52a293eb4389
de Castro, Gabriela
157d4355-6a45-410b-b1db-bc9a74515a0a
Cardoso, João
709f6b15-e7e4-4352-9d74-b516f60379e1
Ovidio, Paula
766b939d-1d1c-4b1e-bb4a-2a08f98d2843
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Jordao, Alceu
3b299005-a0bf-4fb6-8850-8a5258178203

Bezan, Priscila, Holland, Héric, de Castro, Gabriela, Cardoso, João, Ovidio, Paula, Calder, Philip and Jordao, Alceu (2018) High dose of a conjugated linoleic acid mixture increases insulin resistance in rats fed either a low fat or a high fat diet. Experimental and Clinical Endocrinology and Diabetes. (doi:10.1055/s-0043-118348).

Record type: Article

Abstract

Obesity and related diseases are becoming more prevalent. Conjugated linoleic acid (CLA) might be a useful coadjutant treatment helping to decrease fat mass. However, the precise impact of CLA is unclear because the decreased body fat mass is followed by an increase in insulin resistance. This study aimed to evaluate some of the consequences of a high dose of CLA in rats fed a normal low fat or a high fat diet for 30 days. Male Wistar rats were separated into 4 groups (each n = 10): Control group receiving 7% fat (soybean oil); CLA group receiving 4% soybean oil and 3% CLA mixture; animal fat (AF) group, receiving 45% fat (lard); and animal fat plus CLA (AF+CLA) group, receiving 42% lard and 3% CLA mixture. The CLA mixture contained 39.32 mole% c9,t11-CLA and 40.50 mole% t10,c12-CLA. After 30 days, both CLA groups (CLA and AF+CLA groups) developed insulin resistance, with an increase in glucose in the fasting state and in an insulin tolerance test. The CLA group had increased liver weight and percentage of saturated fatty acids in liver and adipose tissue. Feeding the high fat diet resulted in increased hepatic triacylglycerol accumulation and this was exacerbated by dietary CLA. It is concluded that a high dose of CLA mixture increases insulin resistance and exacerbates hepatic steatosis when combined with a high fat diet.

Text Experimental and Clinical Endocrinology & Diabetes - Accepted Manuscript
Restricted to Repository staff only until 14 November 2018.
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More information

Accepted/In Press date: 14 August 2017
e-pub ahead of print date: 31 January 2018

Identifiers

Local EPrints ID: 413207
URI: https://eprints.soton.ac.uk/id/eprint/413207
ISSN: 0947-7349
PURE UUID: b16a6676-3527-4609-9af8-0e9f08e465ee

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Date deposited: 17 Aug 2017 16:30
Last modified: 16 May 2018 16:30

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Contributors

Author: Priscila Bezan
Author: Héric Holland
Author: Gabriela de Castro
Author: João Cardoso
Author: Paula Ovidio
Author: Philip Calder
Author: Alceu Jordao

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