The University of Southampton
University of Southampton Institutional Repository

Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat

Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat
Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat

We have previously identified a T lymphocyte protein which binds to a site within the LTR of the human immunodeficiency virus type 1 (HIV-1) and exerts an inhibitory effect on virus gene expression. The palindromic site (site B) recognized by this protein is related to the palindromic binding sites of members of the steroid/thyroid hormone receptor family. Here we characterize the T cell protein binding to this site as a 100 kD protein which is most abundant in T cells and which binds to site B as a 200 kD complex. This protein is distinct from other members of the steroid/thyroid hormone receptor family including the COUP protein which has a closely related DNA binding specificity.

Base Sequence, Binding Sites, Blotting, Northern, COUP Transcription Factor I, DNA-Binding Proteins, HIV Long Terminal Repeat, HeLa Cells, Humans, Molecular Sequence Data, Oligonucleotide Probes, Receptors, Thyroid Hormone, Regulatory Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't
0305-1048
5429-5434
Orchard, K.
794654ab-d6cc-488a-ac11-c9217433c7a2
Lang, G
bcf892e1-e01c-4941-9e81-ab6064d3660b
Collins, Mary
55ecf446-27b3-4fdc-ae00-b6b0c8a666d5
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Orchard, K.
794654ab-d6cc-488a-ac11-c9217433c7a2
Lang, G
bcf892e1-e01c-4941-9e81-ab6064d3660b
Collins, Mary
55ecf446-27b3-4fdc-ae00-b6b0c8a666d5
Latchman, D
0523ed5f-2893-4762-bee1-14d89e2b3cd6

Orchard, K., Lang, G, Collins, Mary and Latchman, D (1992) Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat. Nucleic Acids Research, 20 (20), 5429-5434.

Record type: Article

Abstract

We have previously identified a T lymphocyte protein which binds to a site within the LTR of the human immunodeficiency virus type 1 (HIV-1) and exerts an inhibitory effect on virus gene expression. The palindromic site (site B) recognized by this protein is related to the palindromic binding sites of members of the steroid/thyroid hormone receptor family. Here we characterize the T cell protein binding to this site as a 100 kD protein which is most abundant in T cells and which binds to site B as a 200 kD complex. This protein is distinct from other members of the steroid/thyroid hormone receptor family including the COUP protein which has a closely related DNA binding specificity.

Full text not available from this repository.

More information

Published date: 25 October 1992
Keywords: Base Sequence, Binding Sites, Blotting, Northern, COUP Transcription Factor I, DNA-Binding Proteins, HIV Long Terminal Repeat, HeLa Cells, Humans, Molecular Sequence Data, Oligonucleotide Probes, Receptors, Thyroid Hormone, Regulatory Sequences, Nucleic Acid, Repressor Proteins, T-Lymphocytes, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 413221
URI: https://eprints.soton.ac.uk/id/eprint/413221
ISSN: 0305-1048
PURE UUID: 13ab9a39-46a5-40db-a7ee-061a7a9996a8
ORCID for K. Orchard: ORCID iD orcid.org/0000-0003-2276-3925

Catalogue record

Date deposited: 17 Aug 2017 16:30
Last modified: 14 Mar 2019 01:45

Export record

Contributors

Author: K. Orchard ORCID iD
Author: G Lang
Author: Mary Collins
Author: D Latchman

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×