A palindromic element in the human immunodeficiency virus long terminal repeat binds retinoic acid receptors and can confer retinoic acid responsiveness on a heterologous promoter

A palindromic element (site B) located between bases -328 and -347 (relative to the start site of transcription) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) was shown in a gel mobility shift assay to bind the human retinoic acid receptors (RARs). Greatly enhanced binding to this site was observed in the presence of both RAR and the retinoid X receptor. Retinoic acid responsiveness in F9 cells could be conferred on a thymidine kinase promoter by the presence of single or multiple copies of site B and responsiveness was abolished when this sequence was mutated to a form that could not bind RARs. However, the presence of this sequence did not render the HIV-1 LTR responsive to retinoic acid in F9 cells.

Animals, Base Sequence, Carrier Proteins, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV, HIV Long Terminal Repeat, HeLa Cells, Humans, In Vitro Techniques, Mice, Molecular Sequence Data, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Receptors, Cell Surface, Receptors, Retinoic Acid, Retinoid X Receptors, Transcription Factors, Tretinoin, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't

0894-9255

440-445

Orchard, K

794654ab-d6cc-488a-ac11-c9217433c7a2

Lang, G

bcf892e1-e01c-4941-9e81-ab6064d3660b

Harris, Julian

b301758e-2f08-4ab2-b219-a26708d150dc

Collins, Mary

7aa2c75e-5e6e-42c8-9302-315c848f3999

Latchman, David

0523ed5f-2893-4762-bee1-14d89e2b3cd6

May 1993

Orchard, K

794654ab-d6cc-488a-ac11-c9217433c7a2

Lang, G

bcf892e1-e01c-4941-9e81-ab6064d3660b

Harris, Julian

b301758e-2f08-4ab2-b219-a26708d150dc

Collins, Mary

7aa2c75e-5e6e-42c8-9302-315c848f3999

Latchman, David

0523ed5f-2893-4762-bee1-14d89e2b3cd6

Orchard, K, Lang, G, Harris, Julian, Collins, Mary and Latchman, David (1993) A palindromic element in the human immunodeficiency virus long terminal repeat binds retinoic acid receptors and can confer retinoic acid responsiveness on a heterologous promoter. Journal of Acquired Immune Deficiency Syndromes, 6 (5), 440-445.

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Published date: May 1993

Keywords: Animals, Base Sequence, Carrier Proteins, DNA-Binding Proteins, Gene Expression Regulation, Viral, HIV, HIV Long Terminal Repeat, HeLa Cells, Humans, In Vitro Techniques, Mice, Molecular Sequence Data, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Receptors, Cell Surface, Receptors, Retinoic Acid, Retinoid X Receptors, Transcription Factors, Tretinoin, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't